Genomic and transcriptomic analysis of sacred fig (Ficus religiosa)
Authors
K Ashalatha
The University of Trans-Disciplinary Health Sciences and Technology (TDU), Yelahanka, Bengaluru 560064 India
Kallare Arunkumar
Central Silk Board, Central Muga Eri Research and Training Institute (CMER&TI), Ministry of Textiles Lahdoigarh, Jorhat, Assam 785700 India
Malali Gowda
The University of Trans-Disciplinary Health Sciences and Technology (TDU), Yelahanka, Bengaluru 560064 India
Peepal/Bodhi tree (Ficus religiosa L.) is an important, long-lived keystone ecological species. Communities on the Indian subcontinent have extensively employed the plant in Ayurveda, traditional medicine, and spiritual practices. The Peepal tree is often thought to produce oxygen both during the day and at night by Indian folks. The goal of our research was to produce molecular resources using whole-genome and transcriptome sequencing techniques.
Results
The complete genome of the Peepal tree was sequenced using two next-generation sequencers Illumina HiSeq1000 and MGISEQ-2000. We assembled the draft genome of 406 Mb, using a hybrid assembly workflow. The genome annotation resulted in 35,093 protein-coding genes; 53% of its genome consists of repetitive sequences. To understand the physiological pathways in leaf tissues, we analyzed photosynthetically distinct conditions: bright sunny days and nights. The RNA-seq analysis supported the expression of 26,479 unigenes. The leaf transcriptomic analysis of the diurnal and nocturnal periods revealed the expression of the significant number of genes involved in the carbon-fixation pathway.
Conclusions
This study presents a draft hybrid genome assembly for F. religiosa and its functional annotated genes. The genomic and transcriptomic data-derived pathways have been analyzed for future studies on the Peepal tree.
Supplementary Information
The online version contains supplementary material available at 10.1186/s12864-023-09270-z.
Keywords: Ficus religiosa, Peepal, Bodhi, Hybrid genome, Transcriptome, Carbon-fixation pathway
Author Biography
Malali Gowda, The University of Trans-Disciplinary Health Sciences and Technology (TDU), Yelahanka, Bengaluru 560064 India
The University of Trans-Disciplinary Health Sciences and Technology (TDU), DNA Life Organization, Yelahanka, Bengaluru 560064 India
Click on "Archives" to access the full archive of scientific preprints. You may use the categories and the search functionality to find select preprints you're interested in.
