Preprint / Version 1

Development of ITS sequence-based markers to distinguish Berberis aristata DC. from B. lycium Royle and B. asiatica Roxb.

Authors

  • Subramani Balasubramani Centre for Pharmacognosy, Pharmaceutics and Pharmacology, Institute of Ayurveda and Integrative Medicine (I-AIM), Foundation for Revitalization of Local Health Traditions (FRLHT), 74/2, Jarakabande Kaval, Attur Post, Via Yelahanka, Bangalore, 560106 India
  • Gurinder Goraya Indian Forest Service, Sundernagar, Mandi, 174402 Himachal Pradesh India
  • Padma Venkatasubramanian Centre for Pharmacognosy, Pharmaceutics and Pharmacology, Institute of Ayurveda and Integrative Medicine (I-AIM), Foundation for Revitalization of Local Health Traditions (FRLHT), 74/2, Jarakabande Kaval, Attur Post, Via Yelahanka, Bangalore, 560106 India

Keywords:

Daruharidra, Berberis spp., Quality control, Internal transcribed spacer (ITS), DNA marker

Abstract

The stems of Berberis aristata DC. (Berberidaceae) are used in the South Asian traditional medicine as a key ingredient in formulations for eye care, skin diseases, jaundice, rheumatism and also in diabetes. B. lycium Royle and B. asiatica Roxb. are traded as equivalents of B. aristata. Conventional macro-morphology and microscopic examination does not aid in critically distinguishing the three species. DNA markers were developed by amplifying and sequencing the complete internal transcribed spacer region (ITS1, 5.8S rRNA and ITS2) from the genomic DNA, using universal primers. The markers developed are efficient and reliable in authenticating B. aristata, B. asiatica and B. lycium. These are useful as molecular pharmacognostic tool in quality control of raw drugs. Keywords: Daruharidra, Berberis spp., Quality control, Internal transcribed spacer (ITS), DNA marker

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