Preprint / Version 1

Pharmacognostical and phytochemical evaluation of Vara Asanadi Kwatha

Authors

  • Anju Ramachandran Assistant Professor, Department of Kaya Chikitsa, PNPS Ayurveda Medical College and Research Centre, Parakalai, Kasargod, Kerala, India
  • Shyam Prasad Assistant Professor, Department of Kaya Chikitsa, PNPS Ayurveda Medical College and Research Centre, Parakalai, Kasargod, Kerala, India
  • S Samarakoon Senior Lecturer, Gampaha Wickramarachchi Ayurveda Institute, University of Kelaniya, Yakkala, Sri Lanka
  • H Chandola Professor and Head, Department of Kaya Chikitsa, Institute for Post Graduate Teaching and Research in Ayurveda, Gujarat Ayurved University, Jamnagar, Gujarat, India,
  • C Harisha Head, Pharmacognosy Laboratory, Institute for Post Graduate Teaching and Research in Ayurveda, Gujarat Ayurved University, Jamnagar, Gujarat, India
  • V Shukla Head, Pharmaceutical Chemistry Laboratory, Institute for Post Graduate Teaching and Research in Ayurveda, Gujarat Ayurved University, Jamnagar, Gujarat, India

Keywords:

HPTLC, Pharmacognosy, TLC, Vara Asanadi Kwatha

Abstract

Vara Asanadi Kwatha (VAK) is a compound Ayurvedic formulation prescribed in the management of obesity. Pharmacognostical study counting both macroscopic and powder microscopy of raw drug exposed the quality and genuineness of all the constituents of VAK. Organoleptic features of coarse powder made out of the crude drugs were within the standard range. Specific gravity of the decoction was 1.0185 and pH was 5.5.Total solid content present in the Kwatha was 4.525% w/v, total ash 0.949% w/v, and acid insoluble ash was 0.052% w/v. Iron assay showed the presence of Fe2O3 as 0.065% w/v. Qualitative scrutiny demonstrated the presence of flavonoids and tannis. Thin Layer Chromatography (TLC) and High Performance Thin Layer Chromatography (HPTLC) were carried out after organizing appropriate solvent system in which maximum three spots were distinguished in TLC and nine spots in HPTLC and most of the Rf values were identical when done with different sample extractive methods. This shows the presence of certain definite constituents in the decoction and is helpful for the easy separation of these constituents. Keywords: HPTLC, Pharmacognosy, TLC, Vara Asanadi Kwatha

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