Lack of genetic diversity of a critically endangered important medicinal plant Chlorophytum borivilianum in Central India revealed by AFLP markers
Authors
Niraj Tripathi
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur, 482004 India
Navinder Saini
Vivekananda Parvartiya Krishi Anusandhan Sansthan, Indian Council of Agricultural Research, Almora, 263601 India
Priya Nair
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur, 482004 India
Sharad Tiwari
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur, 482004 India
Keywords:
Accessions, Genetic variation, Polymorphic, Primer
Abstract
Chlorophytum borivilianum is a traditional medicinal plant distributed throughout the tropics and subtropics. In the present investigation, AFLP analysis was used to assess the genetic similarity among 34 accessions. Nine primer sets of AFLP amplified 612 fragments, of which 246 fragments were found to be polymorphic. The average number of polymorphic bands per AFLP primer pair was 27.33. The amplified fragments ranged from 50 base pairs to 600 base pairs. Significant correlation was observed between total number of amplified fragments and polymorphic bands (p > 0.05) per primers. Cluster analysis based on AFLP data revealed limited genetic variation within the thirty four accessions collected from various parts of Central Indian forests.
Keywords: Accessions, Genetic variation, Polymorphic, Primer
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