In vitro biological effects of two anti-diabetic medicinal plants used in Benin as folk medicine
Authors
Fifa Bothon
Clermont University, Université d’Auvergne, IMTV, F-63000, Clermont-Ferrand, France
Eric Debiton
Clermont University, Université d’Auvergne, IMTV, F-63000, Clermont-Ferrand, France
Felicien Avlessi
University of Abomey Calavi, Laboratoire d’étude et de Recherche en chimie Appliquée, Ecole Polytechnique d’Abomey Calavi, Cotonou, Benin
Christiane Forestier
Clermont University, Université d’Auvergne, Laboratoire Microorganismes: Génome et Environnement (LMGE), BP 10448, F-63000, Clermont-Ferrand, France
Jean-Claude Teulade
Clermont University, Université d’Auvergne, IMTV, F-63000, Clermont-Ferrand, France
Dominique Sohounhloue
University of Abomey Calavi, Laboratoire d’étude et de Recherche en chimie Appliquée, Ecole Polytechnique d’Abomey Calavi, Cotonou, Benin
Extracts from Polygonum senegalensis (Polygonaceae) and Pseudocedrela kotschyi (Meliaceae) are two important traditionally used medicinal plants in rural Benin to treat many diseases and notably type 2 diabetes. The aim of the study was to investigate the α-glucosidase inhibition, antioxidant and antibacterial activities of those plants extract: Polygonum senegalensis leaves, and Pseudocedrela kotschyi root.
Methods
Hydro-alcoholic (50%) extracts were analyzed for their phytochemical content and tested for their inhibition potency on α-glucosidase from Saccharomyces cerevisiae. Antioxidant activities were assessed using the DPPH, ORAC, FRAP and DCFH-DA (cell based) assay. Finally, the antibacterial activity was evaluated using MIC determination on four Gram-positive cocci (Bacillus subtilis, Clostridium difficile, Enterococcus faecalis, Staphylococcus aureus), three Gram-negative bacilli (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae), and the yeast Candida albicans.
Results
Each extract presented significant α-glucosidase inhibition and antioxidant activities. Polygonum senegalensis leaf extracts were the most active in each in vitro assay with an IC50 = 1.5 μg/ml for α-glucosidase inhibition and an IC50 = 6.8 μg/ml for DPPH scavenging, - 4.5 μmol Fe II/g of dry matter - 9366 μmol Trolox / g DW - for FRAP and ORAC values, respectively. IC50 = 2.3 μg GA / ml for DCFH-DA assay. Concerning its antibacterial activity, a growth inhibitory effect was observed only against three Gram negative bacilli: B. subtilis, E. faecalis, S. aureus and the yeast C. albicans at high concentration.
Conclusion
The results showed that the semi alcoholic extract of the two studied plants possess α-glucosidase inhibitory activity, antioxidant potency, and low antibacterial effect.
Keywords: Polygonum senegalensis, Pseudocedrela kotschyi, α-glucosidase, Antioxidant, Antibacterial
Author Biographies
Fifa Bothon, Clermont University, Université d’Auvergne, IMTV, F-63000, Clermont-Ferrand, France
University of Abomey Calavi, Laboratoire d’étude et de Recherche en chimie Appliquée, Ecole Polytechnique d’Abomey Calavi, Cotonou, Benin
Eric Debiton, Clermont University, Université d’Auvergne, IMTV, F-63000, Clermont-Ferrand, France
INSERM, UMR 990, IMTV, F-63005, Clermont-Ferrand, France
Christiane Forestier, Clermont University, Université d’Auvergne, Laboratoire Microorganismes: Génome et Environnement (LMGE), BP 10448, F-63000, Clermont-Ferrand, France
CNRS, UMR 6023, LMGE, F-63177, Aubiere, France
Jean-Claude Teulade, Clermont University, Université d’Auvergne, IMTV, F-63000, Clermont-Ferrand, France
INSERM, UMR 990, IMTV, F-63005, Clermont-Ferrand, France
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