Preprint / Version 1

In vitro alpha-amylase inhibition and in vivo antioxidant potential of Amaranthus spinosus in alloxan-induced oxidative stress in diabetic rats

Authors

  • BS Kumar aDepartment of Pharmacognosy, Sri K.V. College of Pharmacy, Chickballapur 562101, Karnataka, India
  • K Lakshman bDepartment of Pharmacognosy, PES College of Pharmacy, Bangalore, Karnataka, India
  • R Nandeesh cDepartment of Pharmacognosy, Sree Siddhagang College of Pharmacy, Tumkur, Karnataka, India
  • PA Kumar dDepartment of Pharmacology, Sri K.V. College of Pharmacy, Chickballapur 562101, Karnataka, India
  • B Manoj eDepartment of Pharmaceutical Chemistry, Sri K.V. College of Pharmacy, Chickballapur 562101, Karnataka, India
  • Vinod Kumar dDepartment of Pharmacology, Sri K.V. College of Pharmacy, Chickballapur 562101, Karnataka, India
  • D Shekar dDepartment of Pharmacology, Sri K.V. College of Pharmacy, Chickballapur 562101, Karnataka, India

Keywords:

Amaranthus spinosus, Alpha amylase, Antioxidant, Alloxan, Diabetic

Abstract

Amaranthus spinosus Linn. (Amaranthaceae), commonly known as “Mulluharivesoppu” in Kannada, is used in the Indian traditional system of medicine for the treatment of diabetes. The present study deals with the scientific evaluation of alpha amylase and the antioxidant potential of methanol extract of A. spinosus (MEAS). The aim of this study was to investigate in vitro alpha-amylase enzyme inhibition by CNPG3 (2-chloro-4-nitrophenol α-d-maltotrioside) and in vivo antioxidant potential of malondialdehyde (MDA), glutathione (GSH), catalase (CAT) and total thiols (TT) in alloxan-induced diabetic rats of a methanolic extract of A. spinosus. Blood sugar was also determined in MEAS-treated alloxan-induced diabetic rats. MEAS showed significant inhibition of alpha-amylase activity and IC50 46.02 μg/ml. Oral administration of MEAS (200 and 400 mg/kg) for 15 days showed significant reduction in the elevated blood glucose, MDA and restores GSH, CAT and TT levels as compared with a diabetic control. The present study provides evidence that the methanolic extract of A. spinosus has potent alpha amylase, anti-diabetic and antioxidant activities. Keywords: Amaranthus spinosus, Alpha amylase, Antioxidant, Alloxan, Diabetic

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