Preprint / Version 1

Evaluating the Antimicrobial Activity of Methonolic Extract of Rhus Succedanea Leaf Gall

Authors

  • Savitri Shrestha Centre for Advanced studies in Biosciences, Jain University, Chamrajpete-560019, Bangalore, Karnataka, India
  • Sundara Subaramaihha Centre for Advanced studies in Biosciences, Jain University, Chamrajpete-560019, Bangalore, Karnataka, India
  • Sujan Subbaiah Centre for Advanced studies in Biosciences, Jain University, Chamrajpete-560019, Bangalore, Karnataka, India
  • Ravi Eshwarappa Department of chemistry, School of graduate studies, Jain University, Bangalore-560002, India
  • Dhananjaya Lakkappa Toxinology Lab, School of Chemical and Biotechnology (SCBT), SASTRA University, Tanjavur-613401, Tamilnadu, India

Keywords:

Antimicrobial, Rhus succedanea, Gall extracts, Phytochemicals, Drugs, Traditional medicine

Abstract

Introduction: The worldwide increased bacterial resistance to antibiotics and the undesirable side effects associated with constant use of synthetic drugs has prompted the search for novel antimicrobial agents, particularly those manufactured from plants. This study is designed to ascertain the antibacterial potential of Rhus succedanea leaf gall extracts on the growth of gram-positive and gram–negative bacteria. Methods: The methanolic and hexane extract of different concentrations (100, 250, and 500 μg/ml) were prepared and their antibacterial efficacy was tested against clinical isolates of Escherichia coli, Salmonella typhi, Micrococcus luteus, and Staphylococcus aureus using agar well diffusion method and the size of inhibition zone was measured in millimeters. Results: The methanol and hexane extracts differed significantly in their antimicrobial activity with methanol extract showing a potent inhibitory activity in the range of 16±2 to 23±1, which was almost equal to the values of ciprofloxacin (25±3), used as a standard. Further, the methanol extract was mostly potent and effective in inhibiting the growth of gram-negative bacteria, namely, E. coli, when compared to gram –positive bacteria stains, which are responsible for antimicrobial activities. The phytochemical screening showed positive results for the presence of steroids, triterpenes, alkaloids, and carbohydrates. Conclusion: The potent antibacterial activity of Rhus succedanea leaf gall extracts indicates its useful therapeutic application against bacterial infection. Furthermore, this study indicates that the extract might be exploited as natural drug for the treatment of infectious diseases and could be useful in understanding the relations between traditional cures and current medications. Keywords: Antimicrobial, Rhus succedanea, Gall extracts, Phytochemicals, Drugs, Traditional medicine

Author Biography

Dhananjaya Lakkappa, Toxinology Lab, School of Chemical and Biotechnology (SCBT), SASTRA University, Tanjavur-613401, Tamilnadu, India

Center for Emerging Technologies, Jain University, Ramanagara, Bangalore 562112, India