Objective
To validate the traditional use of Achyranthes coynei (A. coynei) Sant. as an antimicrobial in treatment of various infectious diseases.
Methods
Leaf extracts of A. coynei obtained through successive solvent extraction using petroleum ether, dichrloromethane, chloroform and methanol were used to screen the antimicrobial activity on five Gram positive, five Gram negative bacteria and two fungi. Minimum inhibitory concentration (MIC) was determined by two fold tube-dilution method.
Results
Methanolic leaf extract was more effective than other three extracts on the tested bacteria. Methanolic extract was efficient on Staphylococcus epidermis, Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa with MIC values (0.62±0.00) mg/mL. The fungal organisms were less susceptible against extracts tested.
Conclusions
These results support the traditional use of leaf extracts of A. coynei as they have antimicrobial potential. Further studies are needed for establishing safety, toxicity and pharmacological activity with phytochemical investigation.
Keywords: Achyranthes coynei, Endemic, Antimicrobial, Tube-dilution, Minimum inhibitory concentration
Author Biographies
Gireesh Ankad, Regional Medical Research Centre, ICMR, Nehru Nagar, Belgaum-590010, India
UGC-DSK-PDF, Department of Biotechnology, Shivaji University, Kolhapur, Maharashtra, India. Tel: +912312609155 Fax: +912312692333 E-mail: [email protected]
Vinayak Upadhya, Regional Medical Research Centre, ICMR, Nehru Nagar, Belgaum-590010, India
UGC-DSK-PDF, Department of Biotechnology, Shivaji University, Kolhapur, Maharashtra, India. Tel: +912312609155 Fax: +912312692333 E-mail: [email protected]
Sandeep Pai, Regional Medical Research Centre, ICMR, Nehru Nagar, Belgaum-590010, India
UGC-DSK-PDF, Department of Biotechnology, Shivaji University, Kolhapur, Maharashtra, India. Tel: +912312609155 Fax: +912312692333 E-mail: [email protected]
Harsha Hegde, Regional Medical Research Centre, ICMR, Nehru Nagar, Belgaum-590010, India
UGC-DSK-PDF, Department of Biotechnology, Shivaji University, Kolhapur, Maharashtra, India. Tel: +912312609155 Fax: +912312692333 E-mail: [email protected]
Subarna Roy, Regional Medical Research Centre, ICMR, Nehru Nagar, Belgaum-590010, India
UGC-DSK-PDF, Department of Biotechnology, Shivaji University, Kolhapur, Maharashtra, India. Tel: +912312609155 Fax: +912312692333 E-mail: [email protected]
Click on "Archives" to access the full archive of scientific preprints. You may use the categories and the search functionality to find select preprints you're interested in.
