Phytochemical Analysis and Evaluation of the Antioxidant, Antiproliferative, Antibacterial, and Antibiofilm Effects of Globularia alypum (L.) Leaves
Authors
Sahar Nouir
Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia
Amal Dbeibia
Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy of Monastir, University of Monastir, Monastir 5000, Tunisia
Rim Bouhajeb
Laboratory of Bioactive Natural Substances and Biotechnology Research, Faculty of Dental Medicine of Monastir, University of Monastir, Monastir 5000, Tunisia
Houda Haddad
Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia
Amani Khélifa
Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia
Lotfi Achour
High Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia
Mariem Ghardallou
Department of Community Medicine, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia
Amira Zaïri
Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia
Globularia alypum L. (GA) is a Mediterranean plant of the Globulariaceae family which is widely used in traditional Tunisian medicine. The main goal of this study was to evaluate the phytochemical composition, antioxidant, antibacterial, and antibiofilm activities, and the antiproliferative potential of different extracts of this plant. The identification and the quantification of the different constituents of extracts were determined using gas chromatography–mass spectrometry (GC-MS). The antioxidant activities were evaluated using spectrophotometric methods and chemical tests. The antiproliferative study was based on the use of colorectal cancer SW620 cells, including an antibacterial assessment with the microdilution method and analysis of the antibiofilm effects via the crystal violet assay. All extracts presented several components, mainly sesquiterpenes, hydrocarbon, and oxygenated monoterpenes. The results revealed that the maceration extract had the most important antioxidant effect (IC50 = 0.04 and 0.15 mg/mL), followed by the sonication extract (IC50 = 0.18 and 0.28 mg/mL). However, the sonication extract demonstrated significant antiproliferative (IC50 = 20 µg/mL), antibacterial (MIC = 6.25 mg/mLand MBC > 25 mg/mL), and antibiofilm (35.78% at 25 mg/mL) properties against S. aureus. The results achieved confirm the important role of this plant as a source of therapeutic activities.
Keywords: Globularia alypum (L.), phenolic compounds, antioxidant activity, antiproliferative activity, antibacterial activity, antibiofilm activity
Author Biographies
Houda Haddad, Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia
High Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia
Amani Khélifa, Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia
High Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia
Amira Zaïri, Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia
High Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia
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