The Effect of Turmeric (Curcuma longa) Extract on the Functionality of the Solute Carrier Protein 22 A4 (SLC22A4) and Interleukin-10 (IL-10) Variants Associated with Inflammatory Bowel Disease
Elaine Burgess
Nutrigenomics New Zealand, Plant and Food Research Ltd., Private Bag 92169, Auckland Mail Centre, Auckland 1142, New Zealand; E-Mails: [email protected] (E.J.B.); [email protected] (N.B.P.)
Nigel Perry
Nutrigenomics New Zealand, Plant and Food Research Ltd., Private Bag 92169, Auckland Mail Centre, Auckland 1142, New Zealand; E-Mails: [email protected] (E.J.B.); [email protected] (N.B.P.)
Inflammatory bowel disease (IBD) is a chronic relapsing disease. Genetic predisposition to the disease reduces an individual’s capacity to respond appropriately to environmental challenges in the intestine leading to inappropriate inflammation. IBD patients often modify their diet to mitigate or reduce the severity of inflammation. Turmeric (Curcuma longa L., Zingiberaceae) has historically been used in Chinese, Hindu, and Ayurvedic medicine over several centuries to treat inflammatory disorders. To understand how turmeric may influence the consequences of a genetic predisposition to inappropriate inflammation, we used HEK293 cells to examine the in vitro capacity of turmeric extract and fractions to affect the functionality of two gene variants, solute carrier protein 22 A4 (SLC22A4, rs1050152) and interleukin-10 (IL-10, rs1800896) associated with IBD. We found that a turmeric extract and several chromatographically separated fractions beneficially affected the variants of SLC22A4 and IL-10 associated with IBD, by reducing inappropriate epithelial cell transport (SLC22A4, 503F) and increasing anti-inflammatory cytokine gene promoter activity (IL-10, −1082A). The effect of turmeric on the IL-10 variant was strongly associated with the curcumin content of the extract and its fractions.
Keywords: turmeric, curcumin, IL-10, inflammatory bowel disease, SLC22A4, OCTN1, nutrigenomics
Nutrigenomics New Zealand, Plant and Food Research Ltd., Private Bag 92169, Auckland Mail Centre, Auckland 1142, New Zealand; E-Mails: [email protected] (E.J.B.); [email protected] (N.B.P.)
Nutrigenomics New Zealand, Plant and Food Research Ltd., Private Bag 92169, Auckland Mail Centre, Auckland 1142, New Zealand; E-Mails: [email protected] (E.J.B.); [email protected] (N.B.P.)
The Riddet Institute, Massey University, Palmerston North 4442, New Zealand
Elaine Burgess, Nutrigenomics New Zealand, Plant and Food Research Ltd., Private Bag 92169, Auckland Mail Centre, Auckland 1142, New Zealand; E-Mails: [email protected] (E.J.B.); [email protected] (N.B.P.)
Department of Chemistry, University of Otago, P.O. Box 56, Dunedin 9016, New Zealand
Nigel Perry, Nutrigenomics New Zealand, Plant and Food Research Ltd., Private Bag 92169, Auckland Mail Centre, Auckland 1142, New Zealand; E-Mails: [email protected] (E.J.B.); [email protected] (N.B.P.)
Department of Chemistry, University of Otago, P.O. Box 56, Dunedin 9016, New Zealand
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