Wound healing potential of Vakeri fortified KampillakadiTaila
Authors
Pranjali Dhawal
aAnimal Biotechnology and Biochemistry Division, Kelkar Education Trust's Scientific Research Centre, V. G. Vaze College Campus, Mithagar Road, Mulund East, Mumbai, Maharashtra, 400081, India
Milind Gharpure
bThinq Pharma Cro Ltd A30, Rd Number 10, Wagle Estate, MIDC, Thane West, Thane, Maharashtra 400604, India
Minal Joshi
cMicrobiology Divison, Kelkar Education Trust's Scientific Research Centre, V. G. Vaze College Campus, Mithagar Road, Mulund East, Mumbai, Maharashtra, 400081, India
Rummana Khan
cMicrobiology Divison, Kelkar Education Trust's Scientific Research Centre, V. G. Vaze College Campus, Mithagar Road, Mulund East, Mumbai, Maharashtra, 400081, India
Sidhivinayak Barve
dKelkar Education Trust's Scientific Research Centre, V. G. Vaze College Campus, Mithagar Road, Mulund East, Mumbai, Maharashtra, 400081, India
Traditional medicine in form of decoctions has been known for ages to possess wound healing abilities. One such traditional formulation mentioned in Indian literature Charak Samhita Chikitsa Sthanam is Kampillakadi Taila and tremendous information is available on its implication in the treatment of skin cuts and wounds, diseases, or bacterial infections. This research paper focuses on studying the wound healing property of one such herbal proprietary formulation known as a wound healing oil, derived from Kampillakadi Taila fortified with root extract of Wagatea spicata (VIKHPF).
Objective
The current research is aimed at studying chemical profiling, antioxidant activity, antimicrobial efficacy, in-vitro cell proliferating, and in-vitro wound healing activity of this VKHPF.
Materials and methods
The chemical characterization of VKHPF was done by gas chromatography- fatty acid methyl esters GC-FAME analysis for lipid analysis and gas chromatography high-resolution mass spectrometry (GC-HRMS)for revealing its chemical constituents. Proliferation and migration are two underlying mechanisms involved in the healing of wounds. Hence, in-vitro studies such as cell proliferation assay and in-vitro scratch test on NIH/3T3 mice fibroblast cell line were conducted were used to determine in-vitro wound healing capacity of VKHPF. The oil was also tested for antioxidant effect (DPPH assay) and anti-microbial potential (Time kill test).
Results
The GC-HRMS and GC-FAME analyses revealed rich medicinally important fatty acids and vitamins were present in VKHPF, such as oleic acid, hexadecanoic acid, squalene, α, γ-tocopherol, γ-sitosterol, and benzoic acid. VKHPF at 0.5 mg/ml in media without serum showed 164.00 ± 0.011% cell viability with 64.00% cell proliferation in contrast to media containing serum (100% cell viability). At the same concentration, the wound closure was 98% for VKHPF. The oil sample possessed antioxidant activity with an IC50 value of 3.5 mg/ml and antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa when tested using Time Kill Activity.
Conclusion
This study is the first to report the use of Vakeri fortified Kampillakadi Taila herbal proprietary formulation (VKHPF) in in-vitro wound healing and the present data suggest that it can form a part of modern medicine.
Keywords: Kampillakadi Taila, Wound healing, Scratch assay, Vakeri, DPPH, Antimicrobial
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