Preliminary pharmacognostic screening of Achyranthes coynei stem
Authors
Vinayak Upadhya
Herbal Medicine Division, Regional Medical Research Centre, Indian Council of Medical Research, Belgaum, Karnataka, India
Gireesh Ankad
Herbal Medicine Division, Regional Medical Research Centre, Indian Council of Medical Research, Belgaum, Karnataka, India
Sandeep Pai
Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, Indian Council of Medical Research, Belgaum, Karnataka, India
Shruti Hegde
Department of Postgraduate Studies in Botany, Karnatak University, Dharwad, Karnataka, India
Harsha Hegde
Herbal Medicine Division, Regional Medical Research Centre, Indian Council of Medical Research, Belgaum, Karnataka, India
Achyranthes coynei is a rare, endemic perennial shrub reported from Karnataka and Maharashtra states of India. The plant is used to treat various disorders by folk healers and was proven to have antimicrobial and antioxidant properties. The present study was undertaken to evaluate microscopic and macroscopic characters of A. coynei stem, along with its physicochemical parameters. ProgRes® CapturePro and Microsoft Excel were used for statistical analysis. Perennial, shrubby nature and woody stem were the distinguishing morphological characters observed. Transverse section (TS) illustrated quadrangular outline of the stem and showed the presence of two types of trichomes on the thick-walled epidermis. TS also showed number of rosette calcium oxalates crystals; prismatic and microsphenoid crystals; conjoint, collateral open secondary vascular bundles; and two amphixylic medullary bundles in the pith. Ash and extractive values, micro and macro elements and nutritive factors were estimated in the present study. The presence of alkaloids, saponins and triterpenoids were observed in preliminary phytochemical screening. High-performance thin layer chromatographic analysis yielded different bands and also indicated the presence of oleanolic acid. The studied parameters for A. coynei stem will be useful for identification and authentication of the plant material.
Keywords: Achyranthes coynei, Amaranthaceae, endemic, high-performance thin layer chromatography, pharmacognosy, physicochemistry
Author Biography
Harsha Hegde, Herbal Medicine Division, Regional Medical Research Centre, Indian Council of Medical Research, Belgaum, Karnataka, India
Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, Indian Council of Medical Research, Belgaum, Karnataka, India
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