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Quantitative estimation of total tannin, alkaloid, phenolic, and flavonoid content of the root, leaf, and whole plant of Byttneria herbacea Roxb

Authors

  • Tarun Sharma Department of Dravyaguna Vigyana, National Institute of Ayurveda, Deemed to be University, Jaipur, India
  • Rakesh Gamit Department of Chemistry, Shri C.N.P.F. Arts and D.N. Science College, Dabhoi, Vadodara, India
  • Rabinarayan Acharya Central Council for Research in Ayurvedic Sciences, Janakpuri, New Delhi, India
  • V Shukla Pharmaceutical Chemistry Laboratory, Institute for Teaching and Research in Ayurveda, Jamnagar, Gujarat, India

Keywords:

Byttneria, phenols, Samarakhadyam, Samarakhai, spectrophotometric

Abstract

Byttneria herbacea Roxb., family Sterculiaceae, commonly called as ‘Samarakhai’ in local Odia language, is one of the reputed folklore medicinal herb. Its roots, leaves, and whole plant parts are reported for traditional use in the management of dysentery, sores, cuts, wounds, cholera, leucorrhoea, fractures, and sprains. Aim: The aim of the present work was to assess the total tannin content, total alkaloid content (TAC), total phenolic content (TPC), and total flavonoid content (TFC) in root, leaf, and whole plant of B. herbacea. Materials and methods: The amount of total tannins was analyzed using titrimetric method and total alkaloids by gravimetrical method. TPC was measured using Folin-Ciocalteu’s method and calculated as gallic acid equivalents and the amount of total flavonoids by aluminum chloride colorimetric method and calculated as chrysin equivalents. Results: Tannin content was found maximum in the leaf (8.148% w/w) followed by whole plant (3.886% w/w) and root (1.553% w/w); similarly, TAC in the leaf (2.306% w/w) was more than those in root (0.814% w/w) and whole plant (1.319% w/w). The TPC of the methanolic extract of root (372.33 ± 14.29 mg/g) was more than whole plant (267.33 ± 7.63 mg/g); The TFC of the methanolic extract of leaf (620 ± 50 mg/g) was found maximum followed by root (553.33 ± 28.86 mg/g) and whole plant (536.66 ± 28.86 mg/g). Conclusion: The result of study emphasized presence of tannin, alkaloid, phenol, and flavonoid contents in the root, leaf, and whole plant of B. herbacea where the leaf was found to be richest source. Keywords: Byttneria, phenols, Samarakhadyam, Samarakhai, spectrophotometric

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