The Anti-Acne Potential and Chemical Composition of Knautia drymeia Heuff. and Knautia macedonica Griseb Extracts
Authors
Małgorzata Chrząszcz
Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland; [email protected]
Małgorzata Miazga-Karska
Department of Biochemistry and Biotechnology, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland; [email protected] (M.M.-K.); [email protected] (K.K.)
Katarzyna Klimek
Department of Biochemistry and Biotechnology, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland; [email protected] (M.M.-K.); [email protected] (K.K.)
Michał Dybowski
Department of Chromatography, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie Sklodowska University in Lublin, 20-031 Lublin, Poland; [email protected] (M.P.D.); [email protected] (R.T.)
Rafał Typek
Department of Chromatography, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie Sklodowska University in Lublin, 20-031 Lublin, Poland; [email protected] (M.P.D.); [email protected] (R.T.)
Dorota Tchórzewska
Department of Plant Anatomy and Cytology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland; [email protected]
Katarzyna Szewczyk
Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland; [email protected]
The treatment of acne and other seborrheic diseases has arisen as a significant clinical challenge due to the increasing appearance of multi-drug resistant pathogens and a high frequency of recurrent lesions. Taking into consideration the fact that some Knautia species are valuable curatives in skin diseases in traditional medicine, we assumed that the thus far unstudied species K. drymeia and K. macedonica may be a source of active substances used in skin diseases. The purpose of this study was to evaluate the antioxidant, anti-inflammatory, antibacterial, and cytotoxic activities of their extracts and fractions. An LC-MS analysis revealed the presence of 47 compounds belonging to flavonoids and phenolic acids in both species while the GC-MS procedure allowed for the identification mainly sugar derivatives, phytosterols, and fatty acids and their esters. The ethanol as well as methanol-acetone-water (3:1:1) extracts of K. drymeia (KDE and KDM) exhibited great ability to scavenge free radicals and good capacity to inhibit cyclooxygenase-1, cyclooxygenase-2, and lipoxygenase. Moreover, they had the most favorable low minimal inhibitory concentration values against acne strains, and importantly, they were non-toxic toward normal skin fibroblasts. In conclusion, K. drymeia extracts seem to be promising and safe agents for further biomedical applications.
Keywords: Knautia, Caprifoliaceae, skin disorders, antibacterial, anti-inflammatory, antioxidant, cytotoxicity, LC-MS, GC-MS
Author Biographies
Małgorzata Chrząszcz, Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland; [email protected]
Conceptualization, Formal analysis, Data curation, Writing – original draft
Małgorzata Miazga-Karska, Department of Biochemistry and Biotechnology, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland; [email protected] (M.M.-K.); [email protected] (K.K.)
Methodology, Formal analysis, Data curation, Writing – review & editing
Katarzyna Klimek, Department of Biochemistry and Biotechnology, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland; [email protected] (M.M.-K.); [email protected] (K.K.)
Michał Dybowski, Department of Chromatography, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie Sklodowska University in Lublin, 20-031 Lublin, Poland; [email protected] (M.P.D.); [email protected] (R.T.)
Methodology, Formal analysis, Data curation
Rafał Typek, Department of Chromatography, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie Sklodowska University in Lublin, 20-031 Lublin, Poland; [email protected] (M.P.D.); [email protected] (R.T.)
Methodology, Formal analysis, Data curation
Dorota Tchórzewska, Department of Plant Anatomy and Cytology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland; [email protected]
Supervision
Katarzyna Szewczyk, Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland; [email protected]
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