Preprint / Version 1

In vitro cytotoxic and in silico activity of piperine isolated from Piper nigrum fruits Linn

Authors

  • Padmaa Paarakh Department of Pharmacognosy, The Oxford College of Pharmacy, 6/9, I Cross, Begur Road, Hongasandra, Bangalore, 560068 Karnataka India
  • Dileep Sreeram R & D [Phytochemistry], Natural Remedies Private limited, Bangalore, India
  • Shruthi D Microbiology and Cell Biology Department, Indian Institute of Science, Bangalore, Karnataka India
  • Sujan Ganapathy Research and Development Centre, Olive LifeSciences Pvt. Ltd., Nelamangala, Bangalore, 562123 Karnataka India

Keywords:

In vitro cytotoxic activity, In silico docking studies, Isolation, Piperine, Piper nigrum

Abstract

Piper nigrum [Piperaceae], commonly known as black pepper is used as medicine fairly throughout the greater part of India and as a spice globally. Purpose To isolate piperine and evaluate in vitro cytotoxic [antiproliferative] activity and in silico method. Methods Piperine was isolated from the fruits of P.nigrum. Piperine was characterized by UV,IR, 1H-NMR, 13C-NMR and Mass spectrum. Standardization of piperine was done also by HPTLC fingerprinting. In vitro cytotoxic activity was done using HeLa cell lines by MTT assay at different concentrations ranging from 20 to 100 μg/ml in triplicate and in silico docking studies using enzyme EGFR tyrosine kinase. Results Fingerprinting of isolated piperine were done by HPTLC method. The IC50 value was found to be 61.94 ± 0.054 μg/ml in in vitro cytotoxic activity in HeLa Cell lines. Piperine was subjected to molecular docking studies for the inhibition of the enzyme EGFR tyrosine kinase, which is one of the targets for inhibition of cancer cells. It has shown −7.6 kJ mol−1 binding and 7.06 kJ mol−1 docking energy with two hydrogen bonds. Conclusion piperine has shown to possess in vitro cytotoxic activity and in silico studies. Keywords: In vitro cytotoxic activity, In silico docking studies, Isolation, Piperine, Piper nigrum

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