Evaluation of anticancer properties of a decoction containing Adenanthera pavonina L. and Thespesia populnea L.
Authors
Indeewari Lindamulage
Department of Biochemistry and Molecular Biology, University of Colombo, Colombo, Sri Lanka
Preethi Soysa
Department of Biochemistry and Molecular Biology, University of Colombo, Colombo, Sri Lanka
Keywords:
Adenanthera pavonina, Thespesia populnea, Apoptosis, Traditional decoction, Cytotoxicity
Abstract
A decoction composed of Adenanthera pavonina L. and Thespesia populnea L. is currently being used in the treatment of cancer patients.
Methods
Lactate Dehydrogenase (LDH) release, (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) MTT, and Sulforhodamine B (SRB) assays were carried out to study cytotoxicity and anti-proliferative activity against the HEp-2 cells, 24 h post-treatment with the decoction.
Results
The mean (± SD) values of EC50 were 195.50 (±40.68), 120.02 (±29.82) and 77.06 (±8.80) μg/ml for LDH, MTT, and SRB assays respectively. These results strongly correlate the morphological changes observed in cells treated with the decoction. Induction of apoptosis was visualized by fluorescence microscopy stained with ethidium bromide/acridine orange dye mix. In addition, brine shrimp lethality assay showed an EC50 value at a higher concentration (1.96 mg/mL).
Conclusions
These results suggest that the decoction prepared with Adenanthera pavonina L. and Thespesia populnea L. exhibits anti-proliferative activity and induces apoptosis on the HEp-2 cancer cells but no toxicity against Artemia salina.
Keywords: Adenanthera pavonina, Thespesia populnea, Apoptosis, Traditional decoction, Cytotoxicity
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