Preprint / Version 1

Sleep-promoting activity of amylase-treated Ashwagandha (Withania somnifera L. Dunal) root extract via GABA receptors

Authors

  • Chun Park aBK21FOUR R&E Center for Learning Health Systems, Korea University, Seoul 02841, South Korea
  • Ki-Bae Hong cDepartment of Food Science and Nutrition, Jeju National University, Jeju 63243, South Korea
  • Hyung Suh aBK21FOUR R&E Center for Learning Health Systems, Korea University, Seoul 02841, South Korea
  • Yejin Ahn bDepartment of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, South Korea

Keywords:

Antagonist, Electroencephalogram, GABAA receptor, NREM sleep, Withania somnifera

Abstract

Ashwagandha (Withania somnifera L. Dunal), an Indian medicinal plant that has been used for centuries to treat insomnia, exhibits a variety of biological activities, such as improving cognitive function, immunity and anxiety. In this study, the effect of enzyme-treated Ashwagandha root extract (EA) and on sleep was evaluated using rodent models. Starch contained in the Ashwagandha root extract was removed by amylase treatment to prepare EA. To evaluate the sleep-promoting activity of EA, a pentobarbital-induced sleep test and electroencephalogram analysis were performed. In addition, the sleep-promoting mechanism of EA was elucidated by analyzing the expression of sleep-related receptors. In the pentobarbital-induced sleep test, EA dose-dependently increased sleep duration. Additionally, electroencephalogram analysis revealed that EA significantly increased δ-wave and non-rapid eye movement sleep times, which are involved in deep sleep, thereby improving sleep quality and quantity. EA also effectively relieved caffeine-induced insomnia symptoms. Furthermore, the γ-aminobutyric acid (GABA) content in the brain and mRNA and protein expression of GABAA, GABAB1, and serotonin receptors were significantly increased by EA compared to the normal group. In particular, EA showed sleep-promoting activity by binding to various GABAA receptor sites. Collectively, EA exhibited sleep-promoting activity through the GABAergic system and may be used as a functional material to improve sleep deprivation. Keywords: Antagonist, Electroencephalogram, GABAA receptor, NREM sleep, Withania somnifera

Author Biographies

Chun Park, aBK21FOUR R&E Center for Learning Health Systems, Korea University, Seoul 02841, South Korea

bDepartment of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, South Korea

Hyung Suh, aBK21FOUR R&E Center for Learning Health Systems, Korea University, Seoul 02841, South Korea

bDepartment of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, South Korea

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