Preprint / Version 1

Protective Effect of Bioactivity Guided Fractions of Ziziphus jujuba Mill. Root Bark against Hepatic Injury and Chronic Inflammation via Inhibiting Inflammatory Markers and Oxidative Stress

Authors

  • Raghuram Kandimalla Drug Discovery Laboratory, Institute of Advanced Study in Science and Technology, Guwahati, India
  • Suvakanta Dash Girijananda Chowdhury Institute of Pharmaceutical Science, Guwahati, India
  • Sanjeeb Kalita Drug Discovery Laboratory, Institute of Advanced Study in Science and Technology, Guwahati, India
  • Bhaswati Choudhury Drug Discovery Laboratory, Institute of Advanced Study in Science and Technology, Guwahati, India
  • Sandeep Malampati School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China
  • Kasturi Kalita Department of Pathology, Hayat Hospital, Guwahati, India
  • Bhupalee Kalita Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India
  • Rajlakshmi Devi Drug Discovery Laboratory, Institute of Advanced Study in Science and Technology, Guwahati, India
  • Jibon Kotoky Drug Discovery Laboratory, Institute of Advanced Study in Science and Technology, Guwahati, India

Keywords:

liver toxicity, antioxidant, cytokines, inflammation, oxidative stress

Abstract

The tribal communities of North Eastern India rely on herbal medicine to cure various disease conditions. Ziziphus jujuba Mill. (Rhamnaceae) is one of such medicinal plants used for curing liver ailments, insomnia, anemia, diarrhea, diabetic complications, cancer, and loss of appetite. The present study was aimed to describe the protective ability of Z. jujuba root bark (ZJRB) against hepatic injury and chronic inflammation. Bioactivity guided fractionation of Z. jujuba methanol extract (ZJME) was performed using different solvents of increasing polarity viz. hexane (ZJHF), chloroform (ZJCF), ethyl acetate (ZJEAF), water (ZJWF), and residue (ZJMR). In vitro antioxidant results revealed that both ZJME and ZJWF possess strong antioxidant activity among all the fractions and mother extract tested. Further, ZJME and ZJWF showed significant protection against CCl4 intoxicated HepG2 cell lines by means of increased cell viability and decreased LDH levels compared to control group. ZJME at 200, 400 mg/kg and ZJWF at 50, 100 mg/kg inhibited the lipid peroxidation and significantly restored the liver function markers (AST, ALT, ALP, LDH, SOD, and CAT) and cytokine levels (TNF-α, Il-1β, and Il-10) in CCl4 induced acute liver damage in rats. All the results were comparable with standard drug silymarin which was further confirmed by histopathology analysis of liver. Similarly, inflammation and increase inflammatory cytokines levels of carrageenan induced paw edema in rats have been refurbished to normal levels on par with the standard drug indomethacin. ZJWF demonstrated potent response than ZJME in all the biological tests conducted. The results of the study signify the ability of ZJRB as good therapeutic agent for liver toxicity and chronic inflammation. Keywords: liver toxicity, antioxidant, cytokines, inflammation, oxidative stress

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