Understanding the effects of Abhraka Bhasma on genotoxicity and its DNA repair potential in mouse model
Authors
Divya Kulala
aDepartment of Ageing Research, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
Keshava Prasad
bDepartment of Cell and Molecular Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
Poojitha Reddy
aDepartment of Ageing Research, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
Swathi Maruthiyodan
aDepartment of Ageing Research, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
Manjunath Joshi
aDepartment of Ageing Research, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
Kapaettu Satyamoorthy
bDepartment of Cell and Molecular Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
Kanive Guruprasad
aDepartment of Ageing Research, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
Keywords:
Genotoxicity, DNA repair, Abhraka Bhasma, Chromosomal aberrations, reproductive toxicity
Abstract
Metal toxicity is of major concern to human health. The metals may modulate molecular mechanisms of various pathways. Rasashastra, the branch of Ayurveda, narrates the properties, unique preparation, processing techniques, and therapeutic uses of minerals. The use of herbal metallic preparations has evoked concern for their potential to produce toxicity, interest in efficacy as therapeutic agents and safety related issues. Abhraka Bhasma, is one such incinerated herbo-metallic preparation of mica, widely used by traditional medicine practitioners. Although there are reports of Abhraka Bhasma on beneficial effects, clear evidence is lacking on the effect of Abhraka Bhasma on genotoxicity and DNA repair.
Objective
The present study aims to understand the effects of Abhraka Bhasma on geno toxicity, DNA repair, and other mechanisms in the mice test model.
Material and methods
The experiments were conducted in in vivo Swiss albino mice. The acute oral toxicity was performed as per the OECD guidelines. The mice were treated with Abhraka Bhasma (120 or 360 mg/kg body weight) for 7 days. They were then challenged with ethyl methanesulfonate and the DNA repair was analyzed.
Results
The data obtained indicated that the Abhraka Bhasma is not a genotoxic and reproductive toxic formulation. The selected higher concentration of Abhraka Bhasma showed a protective role against ethyl methanesulfonate induced chromosomal damages and enhanced constitutive DNA base excision repair in mice.
Conclusion
The anti-oxidant, potentiation of DNA repair and hematinic properties of Abhraka Bhasma may be attributed to the synergistic actions of its bioactive components.
Keywords: Genotoxicity, DNA repair, Abhraka Bhasma, Chromosomal aberrations, reproductive toxicity
Author Biographies
Manjunath Joshi, aDepartment of Ageing Research, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
cCentre for Ayurvedic Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
Kapaettu Satyamoorthy, bDepartment of Cell and Molecular Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
cCentre for Ayurvedic Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
Kanive Guruprasad, aDepartment of Ageing Research, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
cCentre for Ayurvedic Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
Click on "Archives" to access the full archive of scientific preprints. You may use the categories and the search functionality to find select preprints you're interested in.
