Comparative antioxidant potential of Withania somnifera based herbal formulation prepared by traditional and non-traditional fermentation processes
Authors
Jagdish Manwar
aDepartment of Pharmaceutical Chemistry, Bharati Vidyapeeth University's Poona College of Pharmacy, Pune, India
Kakasaheb Mahadik
aDepartment of Pharmaceutical Chemistry, Bharati Vidyapeeth University's Poona College of Pharmacy, Pune, India
L Sathiyanarayanan
aDepartment of Pharmaceutical Chemistry, Bharati Vidyapeeth University's Poona College of Pharmacy, Pune, India
Anant Paradkar
bInstitute of Pharmaceutical Innovation, and IRC in Polymer Science and Technology, University of Bradford, Bradford, UK
Sanjay Patil
cDepartment of Alcohol Technology, Vasantdada Sugar Institute, Manjari (BK) Pune, India
Keywords:
Alcohol, withaferine A, Withanolide A, Antioxidant potential
Abstract
Ashwagandharishtha is a liquid polyherbal formulation traditionally prepared by fermentation process using the flowers of Woodfordia fruticosa. It contains roots of Withania somnifera as a major crude drug. Alcohol generated during the fermentation causes the extraction of water insoluble phytoconstituents. Yeasts present on the flowers are responsible for this fermentation.
Methods
Total nine formulations of ashwagandharishtha were prepared by fermentation process using traditional Woodfordia fruticosa flowers (ASG-WFS) and using yeasts isolated from the same flowers. During fermentation, kinetic of alcohol generation, sugar consumption, changes in pH and withanolides extraction were studied. All the formulations were tested for in vitro antioxidant potential by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, hydrogen peroxide scavenging and total reducing power assay. The results were compared with standard ascorbic acid.
Results
Traditional formulation (ASG-WFS) showed the highest activity (p < 0.001) relative to other formulations and standard ascorbic acid. ASG-WFS showed significant (DPPH) free radical scavenging (78.75%) and hydrogen peroxide scavenging (69.62%) at the concentration of 1000 μg/mL and 100 μg/mL, respectively.
Conclusion
Traditional process is the best process for preparing ashwagandharishtha to obtain significant antioxidant activity.
Keywords: Alcohol, withaferine A, Withanolide A, Antioxidant potential
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