Preprint / Version 1

Isolation and Characterization of an Acyclic Isoprenoid from Semecarpus anacardium Linn. and its Antibacterial Potential in vitro: - Antimicrobial Activity of Semecarpus anacardium Linn. Seeds -

Authors

  • Ayyakkannu Purushothaman PG & Research Department of Biochemistry, Mohamed Sathak College of Arts and Science, Tamil Nadu, India
  • Packirisamy Meenatchi PG & Research Department of Biochemistry, Mohamed Sathak College of Arts and Science, Tamil Nadu, India
  • Nallappan Saravanan Department of Zoology, Presidency College (Autonomous), Tamil Nadu, India
  • Muthu Karuppaiah Department of Chemistry, Manomanium Sundaranar University, Tirunelveli, Tamil Nadu, India
  • Ramalingam Sundaram PG & Research Department of Biochemistry, Mohamed Sathak College of Arts and Science, Tamil Nadu, India

Keywords:

acyclic isoprenoid, antibacterial, disk diffusion method, high-resolution mass spectrometry, Semecarpus anacardium Linn

Abstract

Objectives Semecarpus anacardium Linn. is a plant well-known for its antimicrobial, antidiabetic and anti-arthritic properties in the Ayurvedic and Siddha system of medicine. This has prompted the screening of this plant for antibacterial activity. The main aims of this study were to isolate compounds from the plant’s seeds and to evaluate their antibacterial effects on clinical bacterial test strains. Methods The n-butanolic concentrate of the seed extract was subjected to thin layer chromatography (TLC) and repeated silica gel column chromatography followed by elution with various solvents. The compound was identified based on observed spectral (IR, 1H NMR, 13C NMR and high-resolution mass spectrometry) data. The well diffusion method was employed to evaluate the antibacterial activities of the isolated acyclic isoprenoid compound (final concentration: 5 – 15 μg/mL) on four test bacterial strains, namely, Staphylococcus aureus (MTCC 96), Bacillus cereus (MTCC 430), Escherichia coli (MTCC 1689) and Acinetobacter baumannii (MTCC 9829). Results Extensive spectroscopic studies showed the structure of the isolated compound to be an acyclic isoprenoid (C21H32O). Moreover, the isoprenoid showed a remarkable inhibition of bacterial growth at a concentration of 15 μg/mL compared to the two other doses tested (5 and 10 μg/mL) and to tetracycline, a commercially available antibiotic that was used as a reference drug. Conclusion The isolation of an antimicrobial compound from Semecarpus anacardium seeds validates the use of this plant in the treatment of infections. The isolated compound found to be active in this study could be useful for the development of new antimicrobial drugs. Keywords: acyclic isoprenoid, antibacterial, disk diffusion method, high-resolution mass spectrometry, Semecarpus anacardium Linn

Author Biography

Ramalingam Sundaram, PG & Research Department of Biochemistry, Mohamed Sathak College of Arts and Science, Tamil Nadu, India

Scientist Grade- III, Central Research Laboratory, Meenakshi Ammal Dental College and Hospital, Maduravoyal, Tamil Nadu, India

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