Protective Effect of a Polyherbal Traditional Formula Consisting of Rosa damascena Mill., Glycyrrhiza glabra L. And Nardostachys jatamansi DC., Against Ethanol-induced Gastric Ulcer
Authors
Zahra Memariani
a
Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Tayebeh Toliyat
a
Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Ghazaleh Moghaddam
b
Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Gholamreza Amin
a
Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Roja Rahimi
a
Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Asal Yans
e
Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Fariba Khodagholi
e
Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Bagher Minaee
d
Department of Histology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Mannan Hajimahmoodi
b
Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Sharifzadeh
h
Department of Pharmacology and Toxicology, Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
“VARD” formula consisting of Rosa damascena Mill. (Rosaceae) petals, and rhizomes of Glycyrrhiza glabra L. (Papilionaceae) and Nardostachys jatamansi DC. (Valerianaceae), has been proposed for gastric ulcer in Iranian traditional medicine. We investigated the antiulcer activity of each plant separately and in combination. The biochemical and molecular functions of extracts were also evaluated. Each plant hydroalcoholic extract was standardized via determination of total phenolic and flavonoid contents, also via some phenolic compounds determination and specially glycyrrhizic acid in G. glabra by using HPLC. Rats received orally extracts of the plants (20, 40 and 80 mg/Kg) and “VARD” (45 mg/Kg) 1 h before ethanol administration. Two h after receiving ethanol, animals were sacrificed; the stomach was removed for macroscopic and microscopic assessment. Also heme-oxygenase-1, glutathione, and catalase were measured in the gastric tissue of the rats pretreated by “VARD” and dose of 20 mg/Kg of extracts. Among three extracts, R. damascena and G. glabra contained more total phenolic and flavonoid content respectively. Gallic acid was prominent compound in R. damascena. The extracts of R. damascena, G. glabra, and N. jatamansi significantly decreased ulcer index. ED50 values were 8.2, 31.86 and 25.08 mg/Kg respectively. “VARD” significantly decreased ulcer index compared to 20 mg/Kg of G. glabra (p < 0.0001) and N. jatamansi (p < 0.001). Pretreatment with “VARD” and each plant extracts (20 mg/Kg) increased glutathione, catalse and heme-oxygenase-1 significantly (p < 0.05) in comparison with control group. Our findings indicate that “VARD” partly via antioxidant activity can be considered as an effective antiulcer formula.
Key Words: Antioxidant, Glycyrrhiza glabra, Heme-oxygenase-1, Inflammation, Nardostachys jatamansi, Peptic ulcer, Rosa damascena
Author Biographies
Tayebeh Toliyat, a
Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
g
Deptartment of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Science, Tehran, Iran.
Gholamreza Amin, a
Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
f
Department of pharmacognosy, faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Mannan Hajimahmoodi, b
Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
c
Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran.
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