Simultaneous Quantification of Forskolin and Iso-Forskolin in Coleus forskohlii (Wild.) Briq. and Identification of Elite Chemotype, Collected from Eastern Ghats (India)
Authors
Pushpendra Shukla
Division of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India
Ankita Misra
Division of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India
Manish Kumar
Division of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India
Jaichand
Division of Central Instrumentation Facility, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India
Kuldeep Singh
Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh, India
Juber Akhtar
Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh, India
Sharad Srivastava
Division of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India
Pawan Agrawal
National Agricultural Science Fund, ICAR, KAB-I, PUSA Campus, New Delhi, India
Ajay Rawat
Division of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India
Coleus forskohlii is a well-known industrially important medicinal plant, for its high forskolin content.
Objective:
A simple, selective, and sensitive high-performance thin layer chromatography (HPTLC) method was developed and validated for simultaneous quantification of forskolin and iso-forskolin in C. forskohlii germplasm collected from the Eastern Ghats, India.
Materials and Methods:
Chromatographic separation of the targeted marker(s) was obtained on precoated silica plates using toluene: ethyl acetate: methanol (90:30:0.5, v/v/v) as the mobile phase.
Results:
Densitometric quantification of forskolin and iso-forskolin was carried out at 545 nm. Forskolin and iso-forskolin were identified by comparing the ultraviolet spectra of standard and sample track at Rf of 0.64 ± 0.02 and 0.36 ± 0.01, after derivatization with anisaldehyde sulfuric acid reagent. The linearity of both the analytes was obtained in the range of 300–1200 ng/spot with the regression coefficient (R2) of 0.991 and 0.986. Recovery of analyte (s) at three levels, namely, 100, 150, and 200 ng/spot was found to be 100.46% ± 0.29%, 99.64% ± 0.33%, 100.02% ± 0.76% and 99.76% ± 0.62%, 99.56% ± 0.35%, 100.02% ± 0.22%, respectively, for forskolin and iso-forskolin. The content of forskolin and iso-forskolin varies from 0.046% to 0.187% and 0.002% to 0.077%, respectively (dry weight basis), the maximum content of both the markers was found in NBC-31, from Thakurwada, Maharashtra.
Conclusion:
The developed HPTLC method was linear, accurate, and reliable as per the International Council for Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use guidelines. The study aids in the identification of elite chemotype for commercial prospection of industrially viable medicinal crop.
SUMMARY
12 Samples are collected from different locations of the eastern ghat regions
Quantification of two major marker forskolin and iso forskolin
The maximum content of both the markers was found in NBC -31, from Thakurwada, Maharashtra
Identification of elite chemotype of collected samples may be useful for commercial prospection in industries.
Keywords: Chemotype, Coleus forskohlii, forskolin, high-performance thin layer chromatography, iso-forskolin
Author Biography
Pushpendra Shukla, Division of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India
Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh, India
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