Glycyrrhiza glabra HPLC fractions: identification of Aldehydo Isoophiopogonone and Liquirtigenin having activity against multidrug resistant bacteria
Authors
Hazir Rahman
Department of Microbiology, Abdul Wali Khan University Mardan, Mardan, Pakistan
Riaz Ullah
Department of Pharmacognosy and Medicinal Aromatic, and Poisonous Plants Research Center, College of Pharmacy King Saud University, Riyadh, 11451 Saudi Arabia
Mansour Al-Said
Department of Pharmacognosy and Medicinal Aromatic, and Poisonous Plants Research Center, College of Pharmacy King Saud University, Riyadh, 11451 Saudi Arabia
Muhammad Adnan
Department of Botany, Kohat University of Science & Technology, Kohat, Pakistan
Muhammad Qasim
Department of Microbiology, Kohat University of Science & Technology, Kohat, Pakistan
Abdul Tawab
National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan
Abdelaaty Shahat
Department of Pharmacognosy and Medicinal Aromatic, and Poisonous Plants Research Center, College of Pharmacy King Saud University, Riyadh, 11451 Saudi Arabia
Anwar Hussain
Department of Botany, Abdul Wali Khan University, Mardan, Pakistan
Ilyas Khan
Department of Microbiology, Kohat University of Science & Technology, Kohat, Pakistan
Shahid Khan
Department of Zoology, Kohat University of Science & Technology, Kohat, Pakistan
Medicinal plants have been founded as traditional herbal medicine worldwide. Most of the plant’s therapeutic properties are due to the presence of secondary metabolites such as alkaloids, glycosides, tannins and volatile oil.
Methods
The present investigation analyzed the High-Pressure Liquid Chromatography (HPLC) fractions of Glycyrrhiza glabra (Aqueous, Chloroform, Ethanol and Hexane) against multidrug resistant human bacterial pathogens (Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus and Pseudomonas aeruginosa). All the fractions showed antibacterial activity, were subjected to LC MS/MS analysis for identification of bioactive compounds.
Results
Among total HPLC fractions of G. glabra (n = 20), three HPLC fractions showed potential activity against multidrug resistant (MDR) bacterial isolates. Fraction 1 (F1) of aqueous extracts, showed activity against A. baumannii (15 ± 0.5 mm). F4 from hexane extract of G. glabra showed activity against S. aureus (10 ± 0.2 mm). However, F2 from ethanol extract exhibited activity against S. aureus (10 ± 0.3 mm). These active fractions were further processed by LC MS/MS analysis for the identification of compounds. Ellagic acid was identified in the F1 of aqueous extract while 6-aldehydo-isoophiopogonone was present in F4 of hexane extract. Similarly, Liquirtigenin was identified in F2 of ethanol.
Conclusions
Glycyrrhiza glabra extracts HPLC fractions showed anti-MDR activity. Three bioactive compounds were identified in the study. 6-aldehydo-isoophiopogonone and Liquirtigenin were for the first time reported in G. glabra. Further characterization of the identified compounds will be helpful for possible therapeutic uses against infectious diseases caused by multidrug resistant bacteria.
Keywords: Glycyrrhiza glabra, HPLC fractionation, Anti-MDR activity, Ellagic acid 6-aldehydo-isoophiopogonone, Liquirtigenin
Author Biography
Abdelaaty Shahat, Department of Pharmacognosy and Medicinal Aromatic, and Poisonous Plants Research Center, College of Pharmacy King Saud University, Riyadh, 11451 Saudi Arabia
Phytochemistry Department, National Research Centre, P.O. Box 12622, 33 El Bohouth st., Dokki, Giza, Egypt
Click on "Archives" to access the full archive of scientific preprints. You may use the categories and the search functionality to find select preprints you're interested in.
