Shuvra Behera
aSchool of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India
Chandrasekaran Rajasekaran
aSchool of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India
S Payas
aSchool of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India
Devanand Fulzele
bPlant Biotechnology and Secondary Metabolites Section, NABTD Bhabha Atomic Research Centre, Mumbai, 400 094, India
C Doss
aSchool of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India
Ramamoorthy Siva
aSchool of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India
Keywords:
Oldenlandia umbellata, Plant dyes, In vitro flowering
Abstract
Oldenlandia umbellata L. (Indian madder) is an antique Ayurvedic Indian herb and a source of various anthraquinone derivatives. The red dye from its roots has been used in diverse applications since ancient times.
Objectives
To establish reliable and effective protocols for in vitro flowering of O. umbellata.
Materials and methods
For in vitro flowering, organogenic calli were subcultured onto Murashige and Skoog (MS) medium supplemented with various concentrations of Naphthalene acetic acid (NAA) (0.15–1.0 mg/l) and Benzyladenine(BA) (0.5–1.5 mg/l) with and without 0.4% of coconut milk (CM).
Results
The highest number of in vitro flowers (22.8%) and best response (92.73%) was achieved on MS medium supplemented with 0.7 mg/l NAA + 1.5 mg/l BA with 0.4% CM. It was found that MS medium devoid of BA promoted best root development (47.3 per calli) as well as response (100%). It was also observed that when embryogenic calli grown in depletion of required nutrition transferred to fresh media induced more flowering. In vivo and in vitro floral comparative analysis revealed that in vitro flower induction was required for short time duration (20.67 days) than in vivo flower.
Conclusion
To the best of our knowledge, this is the first report on in vitro flowering and this study will help to overcome problems associated with flower development and seed production. As a result, this study may be a potent conservation tool to restore innate population size in its natural habitat.
Keywords: Oldenlandia umbellata, Plant dyes, In vitro flowering
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