Zhong-Qi Bian
Center for Infectious Diseases, Kunming General Hospital, PLA, Kunming 650032, China
Keywords:
Chinese medicinal plants, traditional Chinese medicine (TCM), methicillin-resistant Staphylococcus aureus (MRSA), inhibition zone diameters (IZD), minimal bactericidal concentrations (MIC)
Abstract
The in vitro antimicrobial activities of 30 Chinese medicinal plants were evaluated with reference to the treatment record of infectious diseases in the Traditional Chinese Medicine (TCM) literature. The plant materials were extracted with 80% ethanol and the extracts were primarily screened against conventional clinical pathogens like Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans by the agar diffusion method. Their inhibition zone diameters (IZDs, mm, 50 mg/mL) ranged from <8 to 24. The 21 extracts which showed IZDs ≥10 mm against MSSA were also active against methicillin-resistant S. aureus (MRSA) with lower IZDs of 9.0–18.8 mm. They were further subjected to minimal inhibitory concentration and minimal bactericidal concentration (MIC/MBC, μG/mL) assays, which were 8-2,048/32->2,048 by the standard broth microdilution method. The seven extracts from M. yunnanensis, S. sinensis, G. morella, E. daneillii, M. squamulata, S. arborescens and B. hancei were determined as the most active extracts, with MICs of 8–64 μg/mL. The results were in good agreement with their traditional applications in skin and other infections.
Keywords: Chinese medicinal plants, traditional Chinese medicine (TCM), methicillin-resistant Staphylococcus aureus (MRSA), inhibition zone diameters (IZD), minimal bactericidal concentrations (MIC)
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