Antidiabetic and Antioxidant Properties of Alkaloids from Catharanthus roseus (L.) G. Don
Authors
Soon Tiong
Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mails: [email protected] (S.H.T.); [email protected] (H.H.)
Hazrina Hazni
Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mails: [email protected] (S.H.T.); [email protected] (H.H.)
Won Wong
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mail: [email protected]
Shiau-Chuen Cheah
Faculty of Medicine and Health Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, Cheras 56000 Kuala Lumpur, Malaysia; E-Mail: [email protected]
Khalijah Awang
Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mails: [email protected] (S.H.T.); [email protected] (H.H.)
Catharanthus roseus (L.) G. Don is a herbal plant traditionally used by local populations in India, South Africa, China and Malaysia to treat diabetes. The present study reports the in vitro antioxidant and antidiabetic activities of the major alkaloids isolated from Catharanthus roseus (L.) G. Don leaves extract. Four alkaloids—vindoline I, vindolidine II, vindolicine III and vindolinine IV—were isolated and identified from the dichloromethane extract (DE) of this plant’s leaves. DE and compounds I–III were not cytotoxic towards pancreatic β-TC6 cells at the highest dosage tested (25.0 µg/mL). All four alkaloids induced relatively high glucose uptake in pancreatic β-TC6 or myoblast C2C12 cells, with III showing the highest activity. In addition, compounds II–IV demonstrated good protein tyrosine phosphatase-1B (PTP-1B) inhibition activity, implying their therapeutic potential against type 2 diabetes. III showed the highest antioxidant potential in ORAC and DPPH assays and it also alleviated H2O2-induced oxidative damage in β-TC6 cells at 12.5 µg/mL and 25.0 µg/mL.
Keywords: Catharanthus roseus, alkaloids, β-TC6, C2C12, cytotoxicity, antioxidant, glucose uptake, PTP-1B
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