Preprint / Version 1

Evaluation of anti-urolithiatic potential of ethyl acetate extract of Pedalium murex L. on struvite crystal (kidney stone)

Authors

  • B Kaleeswaran aDepartment of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi, Thanjavur, Tamil Nadu, India
  • S Ramadevi aDepartment of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi, Thanjavur, Tamil Nadu, India
  • R Murugesan aDepartment of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi, Thanjavur, Tamil Nadu, India
  • S Srigopalram bGrassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Cheonan, South Korea
  • T Suman cDepartment of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India
  • T Balasubramanian dDepartment of Pharmacology, Al Shifa College of Pharmacy, Poonthavanam Post, Kizhattur Village, Perinthalmanna, Malappuram Dist, Kerala, India

Keywords:

Struvite crystal, Kidney stone, Urolithiasis, Pedalium murex, Magnesium oxide

Abstract

Pedalium murex (L.) is a traditional herb, commonly used for the treatment of kidney stone related problems. Struvite stone can swiftly grow and become ‘staghorn calculi’ in kidney and its associated areas, which is the most aching urological disorder. The present study investigated the anti-urolithiasis activities of ethyl acetate extract of P. murex L. (EAEP) against struvite crystal. The antibacterial activity of EAEP examined against several urease producing bacteria. It showed the minimum bactericidal concentration (MBC) against Escherichia coli and Staphylococcus aureus (>125). On the other hand, total mass, volume, number, growth rate and dissolution rate of synthesised struvite crystals were observed at different concentrations 0.5%, 0.75%, 1% of EAEP and without EAEP. In which, EAEP addition showed appreciably reduced struvite crystal. Alternatively, MgO (300 mg of EAEP/kg/body weight) induced urolithiasis of Wistar albino rat at the rate of 1 ml for 28 days. Various biochemical parameters in serum, urine and histological analysis of kidney were taken for evaluation. Significant results (p < 0.05) were observed in 1% EAEP (300 mg) treated group than cystone treated group. From the histological study, reduced renal damage and glomerular development were observed. Our experiment, P. murex L. enhances the reducing activity on struvite crystal and prevents the crystal formation both in-vitro and in-vivo. It can be suggesting that P. murex L. and its phyto-components could be used as remedy for the management of kidney stone by dissolving the struvite stone in kidney. Keywords: Struvite crystal, Kidney stone, Urolithiasis, Pedalium murex, Magnesium oxide Abbreviations: EAEP, Ethyl acetate extract of Pedalium murex L.; MgO, Magnesium Oxide; MBC, Minimum Bactericidal Concentration