In vitro anti-inflammatory activity of Ficus racemosa L. bark using albumin denaturation method
Authors
Sharmila Dharmadeva
Department of Bio-Medical Sciences, School of Biomedical Science and Physiology, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton, UK
Lahiru Galgamuwa
Department of Basic Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Sri Lanka
C Prasadinie
Department of Alternative Medicine, Institute of Ayurveda and Alternative Medicine, Panadura, Sri Lanka
Nishantha Kumarasinghe
Department of Pre-Clinical Sciences, Faculty of Medicine, General Sir John Kotelawala Defence University, Colombo, Sri Lanka
Keywords:
Albumin denaturation, anti-inflammatory activity, Ficus racemosa L
Abstract
Introduction:
In Ayurveda, many natural plant compounds are used to inhibit inflammatory pathways for centuries with less side effects. Different parts of Ficus racemosa L. (Udumber) plant are used in Ayurveda for many diseases. However, few studies have been conducted to evaluate pharmacological activities of F. racemosa.
Objective:
The objective of the study was to in vitro analyze anti-inflammatory property of F. racemosa bark using albumin denaturation activity.
Methodology:
F. racemosa bark extraction was performed using cold water and hot water. The concentration gradient of extracts was prepared using egg albumin and phosphate-buffered saline. The extract was incubated in a water bath at 37°C for 15 min and was heated at 70°C for 5 min. One nonsteroidal anti-inflammatory drug and one steroid were used as reference drugs. The percentage inhibition of protein denaturation was calculated.
Results:
The inhibition rate of egg albumin denaturation for water extraction increased gradually with concentration. Significantly higher inhibition was showed in hot water extracts than cold water extracts at the concentration of 0.01 μg/ml and 0.1 μg/ml. In addition, the inhibition rate of water extraction was significantly higher than the reference drugs (P < 0.05).
Conclusion:
Anti-inflammatory activity increases with the concentration of F. racemosa bark. Furthermore, the action of this plant is significantly higher than the reference drugs.
Keywords: Albumin denaturation, anti-inflammatory activity, Ficus racemosa L
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