Preprint / Version 1

Anti-brain cancer activity of chloroform and hexane extracts of Tinospora cordifolia Miers: an in vitro perspective

Authors

  • Anuradha Sharma a Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab 143005, India
  • Sandeep Saggu a Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab 143005, India
  • Rachana Mishra a Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab 143005, India
  • Gurcharan Professor a Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab 143005, India

Keywords:

Glioblastoma, Neuroblastoma, Proliferation, Differentiation, T. cordifolia extract

Abstract

Plants have been suggested as safest source of therapeutic agents, with multi targeted mode of action and least side effects. Tinospora cordifolia, commonly known as Guduchi in India, is one of the most highly valued herbs in Ayurvedic medicine. It possesses potential anti-cancer, anti-inflammatory, hepatoprotective, anti-diabetic, immune-stimulatory and various other beneficial activities. Purpose The present study was aimed to investigate the differentiation inducing potential of chloroform and hexane extracts of T. cordifolia using U87MG glioblastoma and IMR-32 neuroblastoma cell lines as model system. Results Chloroform (Chl-TCE) and hexane (Hex-TCE) extracts significantly reduced the rate of proliferation and induced cell differentiation as evidenced by MTT assay and immunostaining for GFAP and MAP-2 in glioblastoma and neuroblastoma, respectively. Further these extracts increased the expression of stress markers HSP70 and Mortalin and induced senescence. Chloroform and hexane extracts also inhibited the migration of U87MG glioblastoma and IMR-32 neuroblastoma as indicated by wound scratch assay and supported by reduced expression of NCAM. Furthermore these extracts are not toxic to normal cells as they showed no inhibitory effects on primary astrocytic and neuronal cultures. Conclusions The present study suggests that chloroform and hexane extracts of T. cordifolia retard the rate of proliferation, induce differentiation and inhibit migration of human glioblastomas and neuroblastomas, thus may act as potential phytotherapeutic intervention in treatment of neural cancers. Keywords: Glioblastoma, Neuroblastoma, Proliferation, Differentiation, T. cordifolia extract

Author Biography

Rachana Mishra, a Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab 143005, India

b Department of Anatomy and Cell Biology, University of Illinois at Chicago, 808 S. Wood St., Rm 578 MC 512, Chicago, IL 60612, USA

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