Preprint / Version 1

Estimation of boswellic acids in herbal formulations containing Boswellia serrata extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MSe

Authors

  • Kumar Katragunta aCentre for Natural Products and Traditional Knowledge, CSIR-Indian Institute of Chemical Technology, Hyderabad, 500 007, India
  • Bandi Siva aCentre for Natural Products and Traditional Knowledge, CSIR-Indian Institute of Chemical Technology, Hyderabad, 500 007, India
  • Niharika Kondepudi aCentre for Natural Products and Traditional Knowledge, CSIR-Indian Institute of Chemical Technology, Hyderabad, 500 007, India
  • PR Vadaparthi aCentre for Natural Products and Traditional Knowledge, CSIR-Indian Institute of Chemical Technology, Hyderabad, 500 007, India
  • Nadendla Rao bChalapathi Institute of Pharmaceutical Sciences, Lam, Guntur, 522034, India
  • Ashok Tiwari aCentre for Natural Products and Traditional Knowledge, CSIR-Indian Institute of Chemical Technology, Hyderabad, 500 007, India
  • Katragadda Babu aCentre for Natural Products and Traditional Knowledge, CSIR-Indian Institute of Chemical Technology, Hyderabad, 500 007, India

Keywords:

Boswellia serrata, Herbal formulations, Quantification, UPLC-Q-tof-MSe, Boswellic acids, Tirucallic acids

Abstract

Boswellia serrata is a widely used herb in Indian systems of medicine and is well known for its potential medicinal properties. A chromatographic method was developed for the analysis and quantification of six boswellic acid marker compounds, i.e., keto boswellic acid (1), 3-O-Acetyl 11-keto β-boswellic acid (2), ɑ-Boswellic acid (3), β-Boswellic acid (4), 3-O-Acetyl-ɑ-boswellic acid (5) and 3-O-Acetyl-β-boswellic acid (6) in commercial herbal products containing B. serrata as an ingredient. Combining UPLC with Q-Tof-MS/MS makes the better identification of secondary metabolites and adulterants in the herbal formulations containing B. serrata in rapid time using fragmentation approach than the traditional approaches. In this study quantification of boswellic acids with UPLC-PDA method was performed as per the pharmacopeia guidelines. Furthermore, minor phytochemical constituents were identified and characterized with the help of LC-Q-Tof-MS/MS fragmentation data and various isoforms of boswellic acids and tirucallic acids in B. serrata oleo-gum-resin extract were identified. Keywords: Boswellia serrata, Herbal formulations, Quantification, UPLC-Q-tof-MSe, Boswellic acids, Tirucallic acids

Author Biography

Kumar Katragunta, aCentre for Natural Products and Traditional Knowledge, CSIR-Indian Institute of Chemical Technology, Hyderabad, 500 007, India

cCollege of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, 522510, India

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