Microarray analysis of genes from animals treated with a traditional formulation ChandraprabhaVati reveals its therapeutic targets
Authors
Pratheppa Rajagopal
aCentre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
David Chellappan
bCentre for Advanced Research in Indian Systems of Medicine, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
Sriram Sridharan
bCentre for Advanced Research in Indian Systems of Medicine, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
Brindha Pemiah
bCentre for Advanced Research in Indian Systems of Medicine, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
Sridharan Krishnaswamy
cSchool of Chemical & Biotechnology, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
Swaminathan Sethuraman
aCentre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
KalpoondiRajan Sekar
aCentre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
Uma Krishnan
aCentre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
Keywords:
ChandraprabhaVati, Ayurveda, Microarray analysis
Abstract
Traditional medicinal preparations have not received global acceptance, and their therapeutic benefits remain disputed due to lack of scientific evidence on their mechanism of action. Microarray analysis has emerged as a powerful technique that can aid in understanding the complex signaling networks activated by these formulations and thereby assess their beneficial as well as adverse effects.
Aim
The present work aims to investigate the differential influence of ChandraprabhaVati, Ayurvedic formulation used in the treatment of diabetes, anemia, urinary, respiratory, skin and liver disorders.
Materials and methods
The RNA from the liver of rats treated with different doses of ChandraprabhaVati for 28 days was isolated and studied for the genome-wide changes in the expression.
Results
The results revealed several molecular targets that could contribute to the therapeutic effects of ChandraprabhaVati. Several genes have been differentially expressed, among those miRNAs miR-434, miR877, and miRlet7e contribute to the anti-diabetic, anti-fibrotic and anti-inflammatory of CPV. The rejuvenative activity of CPV may be due to the MeOX1 and Upf3b genes. Up-regulation of Hbaa2 gene facilitates the anti-anemic effect. Interestingly gender-specific differential expressions of genes were also observed. Rab3d were found to be altered in female when compared to male animals.
Conclusion
Thus the microarray data for the CPV treated animals has revealed molecular targets that may be responsible for the various known therapeutic effects and also identified new beneficial effects of CPV.
Keywords: ChandraprabhaVati, Ayurveda, Microarray analysis
Author Biographies
Pratheppa Rajagopal, aCentre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
cSchool of Chemical & Biotechnology, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
David Chellappan, bCentre for Advanced Research in Indian Systems of Medicine, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
cSchool of Chemical & Biotechnology, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
Sriram Sridharan, bCentre for Advanced Research in Indian Systems of Medicine, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
cSchool of Chemical & Biotechnology, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
Brindha Pemiah, bCentre for Advanced Research in Indian Systems of Medicine, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
cSchool of Chemical & Biotechnology, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
Swaminathan Sethuraman, aCentre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
cSchool of Chemical & Biotechnology, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
KalpoondiRajan Sekar, aCentre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
cSchool of Chemical & Biotechnology, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
Uma Krishnan, aCentre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
cSchool of Chemical & Biotechnology, SASTRA Deemed-to-be University, Thanjavur, 613 401, Tamil Nadu, India
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