Preprint / Version 1

Asiaticoside Alleviates Cerebral Ischemia-Reperfusion Injury via NOD2/Mitogen-Activated Protein Kinase (MAPK)/Nuclear Factor kappa B (NF-κB) Signaling Pathway

Authors

  • Chunhui Zhang Department of Clinical Nursing, Nursing and Health College of Zhengzhou University, Zhengzhou, Henan, P.R. China
  • Suyan Chen Department of Public Nursing, Nursing and Health College of Zhengzhou University, Zhengzhou, Henan, P.R. China
  • Zhenxiang Zhang Department of Fundamental Nursing, Nursing and Health College of Zhengzhou University, Zhengzhou, Henan, P.R. China
  • Hui Xu Department of Fundamental Nursing, Nursing and Health College of Zhengzhou University, Zhengzhou, Henan, P.R. China
  • Weihong Zhang Department of Basic Medicine, Nursing and Health College of Zhengzhou University, Zhengzhou, Henan, P.R. China
  • Dongqing Xu Rehabilitation Department, Henan Province Hospital of Traditional Chinese Medicine (TCM), Zhengzhou, Henan, P.R. China
  • Beilei Lin Department of Clinical Nursing, Nursing and Health College of Zhengzhou University, Zhengzhou, Henan, P.R. China
  • Yongxia Mei Department of Public Nursing, Nursing and Health College of Zhengzhou University, Zhengzhou, Henan, P.R. China

Keywords:

MeSH Hypoxia-Ischemia, Brain; Medicine, Chinese Traditional; Stroke

Abstract

Cerebral ischemia-reperfusion injury (CIRI) remains a serious health problem. Centella asiatica formulations are used to treat central nervous system disorders. In the present study, asiaticoside, an extract of the plant Centella asiatica, was investigated in CIRI in vivo and vitro. Material/Methods We made a CIRI model in vivo in SD rats treated by middle cerebral artery occlusion, and a cell model of ischemia-reperfusion injury was made in PC12 cells treated by deprivation of oxygen and glucose/restoration. CIRI in vivo was assessed by scores of neurological functions, encephaledema, and cerebral infarction area. Inflammation level and oxidative stress level were detected by the appropriate kits. TUNEL assay was performed for assessment of cell apoptosis and Western blot analysis was performed to assess protein expression levels. CCK8 assay was performed for evaluation of cell survival and flow cytometer was used to detect cell apoptosis in vitro. Results Nervous function injury, brain edema, cell apoptosis, infarct size, apoptosis-related protein expressions, and protein expressions of the NOD2/MAPK/NF-κB signaling pathway in the CIRI model were all reversed by asiaticoside in rats. The cell apoptosis, inflammation level, and oxidative stress level in the model of cerebral ischemia-reperfusion injury were reduced by asiaticoside. The effects of asiaticoside on CIRI were reversed by NOD 2 agonists. Conclusions Asiaticoside showed a protective effect against cerebral ischemia-reperfusion injury via the NOD2/MAPK/NF-κB signaling pathway. These findings are vital for future research on use of asiaticoside in CIRI, providing a new avenue for alleviating CIRI. MeSH Keywords: Hypoxia-Ischemia, Brain; Medicine, Chinese Traditional; Stroke

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