Neuroprotective Effects of Dried Tubers of Aconitum napellus
Authors
Ambreen Shoaib
Department of Clinical Pharmacy, Faculty of Pharmacy, Jazan University, Jazan 45142, Saudi Arabia; [email protected]
Hefazat Siddiqui
Department of Pharmacology, Faculty of Pharmacy, Integral University, Lucknow 226026, India; [email protected]
Rakesh Dixit
Department of Pharmacology, King George Medical University, Lucknow, Uttar Pradesh 226003, India; [email protected]
Sahabjada Siddiqui
Department of Biotechnology, Era’s Lucknow Medical College & Hospital, Era University, Lucknow 226003, India; [email protected]
Badrud Deen
Department of Pharmacology, Faculty of Pharmacy, Integral University, Lucknow 226026, India; [email protected]
Andleeb Khan
Department of Pharmacology & Toxicology, Faculty of Pharmacy, Jazan University, Jazan 45142, Saudi Arabia; [email protected]
Salman Alrokayan
Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; [email protected] (S.H.A.); [email protected] (H.A.K.)
Haseeb Khan
Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; [email protected] (S.H.A.); [email protected] (H.A.K.)
Parvaiz Ahmad
Botany and Microbiology Department, College of Science, King Saudi University, Riyadh 11451, Saudi Arabia
The present study was designed to explore the neuroprotective properties of Aconitum napellus (Ranunculaceae). The plant detoxification was done using either water, or cow or goat milk as per the Ayurvedic shodhana method. The evaluation of the neuroprotective role of A. napellus was performed on diabetic neuropathy induced by streptozotocin in Sprague Dawley (SD) rats. Body mass, blood sugar level, oral glucose tolerance test, hyperalgesia, cold allodynia, motor co-ordination test, and locomotor activity, oxidative biomarkers (TBARS, reduced glutathione, catalase and superoxide dismutase) and sciatic nerve histomorphology were assessed. The in vitro studies were done on human neuroblastoma cell line SHSY-5Y and used an MTT assay to assess the antiproliferative activity of different extracts. Results suggest that the goat milk treated chloroform extract has less percentage of aconitine. After administration of the detoxified chloroform extract to the diabetic animals, there was a significant improvement in the myelination and degenerative changes of the nerve fibers along with behavioral changes (p < 0.05 as compared with diabetic control group). The findings of the in vitro research show an effective neuroprotective role of A. napellus. This suggests that A. napellus should be further investigated for its effect in diabetic pathology.
Keywords: Aconitum napellus, neuropathy, sciatic nerve, histopathology, neuroblastoma cell line, cell viability
Author Biography
Ambreen Shoaib, Department of Clinical Pharmacy, Faculty of Pharmacy, Jazan University, Jazan 45142, Saudi Arabia; [email protected]
Department of Pharmacology, Faculty of Pharmacy, Integral University, Lucknow 226026, India; [email protected]
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