Preprint / Version 1

Effect of Helicanthes elasticus (Desv.) Danser extracts on immune profile of Wistar albino rats

Authors

  • KN Kumar aSiddha Central Research Institute (CCRS), Arignar Anna Govt. Hospital Campus, Arumbakkam, Chennai, India
  • G Reddy aSiddha Central Research Institute (CCRS), Arignar Anna Govt. Hospital Campus, Arumbakkam, Chennai, India
  • Ravi Mundugaru bDepartment of Pharmacology, DM Wayanad Institute of Medical Sciences, Wayanad, India
  • Rajakrishnan Rajagopal cDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia
  • Ahmed Alfarhan cDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia
  • Young Kim dDepartment of Clinical Pharmacology, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea
  • Sae Na eThe Comfort Animal Hospital, Sungbuk-gu, Soonginro-50, Seoul, Republic of Korea
  • Hak-Jae Kim dDepartment of Clinical Pharmacology, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea

Keywords:

Antibody titer, Cell mediated immunity, Germinal cells, Helicanthes elasticus, Sheep red blood cells, White pulp

Abstract

Helicanthes elasticus (Desv.) Danser is a common type of mistletoes of Indian origin. In Indian traditional and folklore medicines the plant is claimed to possess a range of medicinal values such as immunomodulator, anti-diabetic and anti-microbial properties. However, there is no experimental proof for its therapeutic claim. The aqueous and alcoholic extracts of H. elastica were evaluated for its immuno-modulatory effect on antibody formation against sheep red blood cells and on cell mediated immunity of immunological paw edema model. Ethanolic and aqueous extracts have shown dose dependent elevation in the antibody titer value in comparison to control group at 14th and 21st day of sensitization (**p < 0.01). There is a mild to moderate elevation were observed in the immunological paw edema at highest dose (400 mg/kg) during 21st day after sensitization. The histopathological observation shows that there is an increase in the white pulp of spleen and increased cellularity and formation of distinct germinal cells in lymph node. H. elasticus extracts possess marked antibody formation propensity without significant modification on cell mediated immunity. Keywords: Antibody titer, Cell mediated immunity, Germinal cells, Helicanthes elasticus, Sheep red blood cells, White pulp

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