Protective role of Convolvulus pluricaulis on lipid abnormalities in high-fat diet with low dose streptozotocin-induced experimental rat model
Authors
Gaurav Garg
aDepartment of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India
Amol Patil
aDepartment of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India
Rohit Kumar
aDepartment of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India
Alka Bhatia
bDepartment of Experimental Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India
Kripa Kasudhan
aDepartment of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India
Smita Pattanaik
aDepartment of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India
Keywords:
Convolvulus pluricaulis, Streptozotocin, Hypolipidemia, High fat diet
Abstract
The methanolic extract of Convolvulus pluricaulis had earlier shown lipid lowering activity in Triton induced reversible hyperlipidemia model, but, the hypolipidemic activity in irreversible models and hypoglycaemic activity are not investigated so far.
Objective
This study was designed to validate the lipid and glucose-lowering actions of C. pluricaulis methanolic extract (CPME) by using ingredients from the Indian diet for induction of hyperlipidemia and diabetes on experimental rats.
Materials and methods
Experimental animals were divided into four groups having six animals in each group (n = 6). Animals of Group I II, III and IV received - no treatment, 0.9% NaCl, Glipizide (GPZ) 5 mg/kg and CPME 400 mg/kg once daily for two weeks respectively. Animals of all groups except group I were fed a high fat-based Indian diet for 21 days followed by a single STZ (35 mg/kg) i.p. administration in model induction phase. Afterwards, animals were sacrificed, and the pancreas was dissected for histological changes, and blood was collected for measuring lipid parameters, FBS, insulin levels, and HOMA scores.
Results
CPME significantly ameliorate the lipid abnormalities in HFD-STZ-treated experimental model (p < 0.001) but fails to reverse the hyperglycaemia developed in diabetic rats with no protective effect on islet architecture (p > 0.05) as compared to experimental group while, GPZ showed protective effect on both lipid abnormalities and hyperglycemia by modulating the levels of lipid parameters and insulin respectively.
Conclusion
In conclusion, the study confirm that CPME possesses significant hypolipidemic activity but fails to reverse the hyperglycaemia developed in diabetic rats.
Keywords: Convolvulus pluricaulis, Streptozotocin, Hypolipidemia, High fat diet
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