Preprint / Version 1

Cytotoxic and apoptotic inducing activity of Amoora rohituka leaf extracts in human breast cancer cells

Authors

  • Rajesh Singh aCentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
  • Amit Ranjan aCentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
  • Akhileshwar Srivastava bThe National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
  • Monika Singh cSchool of Biomedical Engineering, Indian Institute of Technology-BHU, Varanasi 221005, India
  • Anil Shukla dDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA
  • Neelam Atri eDepartment of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi 221005, India
  • Anurag Mishra fDepartment of Dravyaguna, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
  • Anil Singh fDepartment of Dravyaguna, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
  • Santosh Singh aCentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India

Keywords:

Traditional medicine, Breast cancer, FTIR, HPTLC, Apoptosis, Cell migration

Abstract

Amoora rohituka is described in Ayurveda, an Indian traditional system of medicine for management of disorders of blood, diseases of eye, helminthiasis disease, ulcer, liver disorders and splenomegaly. However, the leaves were not reported to have anticancer properties till date. Objective This study was carried out to evaluate the cytotoxic potential of leaf extracts of Amoora rohituka. Materials and methods The leaves powder was macerated in petroleum ether, ethyl acetate and methanol and evaluated their anticancer activities in vitro. The phytochemical constituents of the active (ethyl acetate) extract were screened by FTIR analysis and phytochemical screening methods. Results The ethyl acetate extract (RLEA) showed the presence of alkaloids, flavonoids, steroids, tannins, saponins and terpenoids. The RLEA exhibited high cytotoxic effect against human breast cancer cells, MCF-7 (IC50 = 9.81 μg/mL) and induced apoptosis by altering nuclear morphology and DNA laddering. Wound healing assays explained the potency of extract to decrease the cell migration. Conclusion The extract of Amoora rohituka leaves exhibited anticancer activity with less toxicity and it could be used for development of alternative drugs in the treatment of human breast cancer. Keywords: Traditional medicine, Breast cancer, FTIR, HPTLC, Apoptosis, Cell migration

Author Biography

Rajesh Singh, aCentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India

fDepartment of Dravyaguna, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India

Downloads