Identification and estimation of bioactive constituents Negundoside, Berberine chloride, and Marmelosin by HPLC and HPTLC for development of quality control protocols for Ayurvedic medicated oil formulation
Authors
Ajay Meena
Regional Ayurveda Research Institute, Aamkho, Gwalior, Madhya Pradesh 474009 India
P Rekha
Captain Srinivasa Murthy Central Ayurveda Research Institute, Arumbakkam, Chennai, Tamil Nadu 600106 India
Ayyam Perumal
Captain Srinivasa Murthy Central Ayurveda Research Institute, Arumbakkam, Chennai, Tamil Nadu 600106 India
R Ilavarasan
Captain Srinivasa Murthy Central Ayurveda Research Institute, Arumbakkam, Chennai, Tamil Nadu 600106 India
Ravindra Singh
Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Government of India, New Delhi, India
N Srikant
Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Government of India, New Delhi, India
K Dhiman
Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Government of India, New Delhi, India
Keywords:
Anu Taila, Medicated oil, Ayurveda, Nasya, HPTLC, HPLC
Abstract
Anu Taila is an ancient medicated oil Ayurvedic preparation that is commonly used for nasya karma. It contains more than 25 herbs and goat milk as per the Ayurvedic Formulary of India (AFI). It strengthens the neck, shoulder, and chest muscles and improves the capacity of sense organs. It delays the aging process and reduces hair fall. Recent studies showed that it is also useful in COVID-19. In the current study, an attempt to develop quality control protocols and evaluate the standardization parameters like refractive index, iodine value, saponification value, peroxide value, acid value, rancidity, HPTLC fingerprint profile along with major bioactive compound and quantification of Berberine chloride, Negundoside, and Marmelosin by HPLC. Establishing quality protocol and standard parameters like physicochemical parameters and estimation of bioactive compounds of this preparation is significant for quality control.
Results
In this study, HPTLC identifies bioactive chemical compounds like Berberine chloride, Marmelosin, Negundoside, glycyrrhizin, and para hydroxybenzoic acid (PHBA), Lupeol, Embelin, and Solasodine, which were present in the Anu Taila formulation. HPLC was used to estimate the bioactive marker compounds Negundoside, Berberine chloride, and Marmelosin were present in the Anu Taila formulation. The quantitative evaluation of Berberine chloride (0.0013%), Marmelosin (0.0366%), Negundoside (0.0086%) is present in Anu Taila formulation.
Conclusion
The study reveals that sufficient quality control parameters were followed during the preparation of the formulation. Physicochemical analysis was carried out as per the guidelines of Ayurvedic Pharmacopeia of India. HPTLC and HPLC profiles generated in this particular study can be considered as a preliminary tool ascertaining the authenticity of Anu Taila.
Keywords: Anu Taila, Medicated oil, Ayurveda, Nasya, HPTLC, HPLC
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