Evaluation of pancreatic regeneration activity of Tephrosia purpurea leaves in rats with streptozotocin-induced diabetes
Authors
Sumit Arora
aDepartment of Pharmacognosy and Phytochemistry, Gurunanak College of Pharmacy, Nari, Nagpur, 440026, Maharashtra, India
Prashant Verma
bResearch and Development Department, Lifespan Industries, Plot No. 49, Phase III, Biotech Park, Genome Valley, Karkapatla, 502279, Telangana, India
Prakash Itankar
cPharmacognosy and Phytochemistry Division, Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Amravati Road, Nagpur, 440033, Maharashtra, India
Satyendra Prasad
cPharmacognosy and Phytochemistry Division, Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Amravati Road, Nagpur, 440033, Maharashtra, India
Kartik Nakhate
dDepartment of Pharmacology, Shri Vile Parle Kelvani Mandal’s Institute of Pharmacy, Dhule, Maharashtra, 424001, India
Background and aim
Flavonoid rich plant Tephrosia purpurea (T. purpurea), commonly known as Sarpunkha has been used in traditional systems of medicine to treat diabetes mellitus. However, its effectiveness in promoting regeneration of pancreas in diabetes has not been investigated. Therefore, the present study was undertaken to evaluate pancreatic β-cells regeneration, antioxidant and antihyperlipidemic potentials of T. purpurea leaves extract, its fractions and main constituent Rutin in diabetic rats.
Experimental procedure
The leaves extract and its fractions were first screened for acute and sub-chronic antidiabetic activity in a dose range of 250–500 mg/kg orally. Further, fractions with potent antidiabetic activity were screened for pancreatic β-cells regeneration activity using histopathological studies and morphometric analysis, which was followed by estimation of biochemical parameters.
Results and conclusion
The most significant antidiabetic, pancreatic regeneration and antihyperlipidemic activity was exhibited by n-butanol soluble fraction of ethanol extract at the dose level of 500 mg/kg. Histopathology revealed that treatment with this fraction improved the β-cell granulation of islets and prevented the β-cells damage which was further confirmed by morphometric analysis. Thus, the present study validated the traditional use of T. purpurea plant in the treatment of diabetes, which might be attributed to pancreatic β-cells regeneration potential of its active constituent Rutin.
Taxonomy (classification by EVISE)
Traditional Medicine; Metabolic Disorder; Experimental Design; Cell Regeneration and Histopathology.
Keywords: Pancreatic β-cell, Diabetes mellitus, Plant extracts, Polyphenol, Antioxidant activity, Biochemical estimation
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