Comprehensive Phytochemical Profiling of Polyherbal Divya-Kayakalp-Vati and Divya-Kayakalp-Oil and Their Combined Efficacy in Mouse Model of Atopic Dermatitis-Like Inflammation Through Regulation of Cytokines
Authors
Acharya Balkrishna
Drug Discovery and Development Division, Patanjali Research Institute, Haridwar, 249 405, Uttarakhand, India
Sudeep Verma
Drug Discovery and Development Division, Patanjali Research Institute, Haridwar, 249 405, Uttarakhand, India
Sachin Sakat
Drug Discovery and Development Division, Patanjali Research Institute, Haridwar, 249 405, Uttarakhand, India
Kheemraj Joshi
Drug Discovery and Development Division, Patanjali Research Institute, Haridwar, 249 405, Uttarakhand, India
Siva Solleti
Drug Discovery and Development Division, Patanjali Research Institute, Haridwar, 249 405, Uttarakhand, India
Kunal Bhattacharya
Drug Discovery and Development Division, Patanjali Research Institute, Haridwar, 249 405, Uttarakhand, India
Anurag Varshney
Drug Discovery and Development Division, Patanjali Research Institute, Haridwar, 249 405, Uttarakhand, India
Purpose
Atopic dermatitis (AD) is a chronic inflammatory disease that varies in signs and symptoms in different individuals. General symptoms include dryness of the skin, itching, and development of red to brownish-gray patches. Divya-Kayakalp-Vati (DKV) and -Oil (DKO) are Indian polyherbal compositions prescribed for treating inflammatory skin diseases. In the present study, we evaluated the anti-inflammatory efficacy of DKV and DKO co-treatment (DKV-O) in ameliorating Oxazolone (OXA)-stimulated AD-like inflammation and pro-inflammatory cytokine release in a Swiss albino mouse model.
Methods
Phytochemical profiling of the DKV and DKO were done using Liquid Chromatography-Mass Spectroscopy (LC-MS) QToF. Swiss albino mice were sensitized for 7 days and treated with OXA in their ear region. Stimulated and control animals were orally treated with DKV and topically with DKO. Anti-inflammatory efficacy of DKV-O was determined in OXA-treated animals through physiological, histopathological, and biochemical parameter analysis.
Results
DKV and DKO formulations individually contained 39 and 59 phytochemicals, respectively. Many of the phytochemicals have been reported to have anti-inflammatory activities. In the OXA-sensitized Swiss albino mice, combined treatment with DKV-O, and separately with Dexamethasone (positive control) significantly reduced the OXA-stimulated ear edema, biopsy weight, and epidermal thickness. DKV-O further reduced OXA-stimulated induction of inflammatory lesions, neutrophil influx, and release of Interleukin (IL)-1β, IL-6, tumor necrosis factor-α, and myeloperoxidase.
Conclusion
Finally, DKV-O co-treatment showed good pharmacological effects in ameliorating AD-like inflammation through the modulation of inflammatory cell influx and release of soluble mediators. Therefore, DKV-O treatment can be used as a suitable polyherbal therapeutic against AD-like inflammatory diseases.
Keywords: inflammation, oxazolone, phytochemical profile, pro-inflammatory cytokines, histopathological analysis, biochemical analysis
Author Biographies
Acharya Balkrishna, Drug Discovery and Development Division, Patanjali Research Institute, Haridwar, 249 405, Uttarakhand, India
Department of Allied and Applied Sciences, University of Patanjali, Patanjali YogPeeth, Haridwar, 249 405, Uttarakhand, India
Anurag Varshney, Drug Discovery and Development Division, Patanjali Research Institute, Haridwar, 249 405, Uttarakhand, India
Department of Allied and Applied Sciences, University of Patanjali, Patanjali YogPeeth, Haridwar, 249 405, Uttarakhand, India
Click on "Archives" to access the full archive of scientific preprints. You may use the categories and the search functionality to find select preprints you're interested in.
