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Isolation and identification of hair growth potential fraction from active plant extract of Blumea eriantha DC grown in Western Ghat of India: In silico study

Authors

  • Somnath Bhinge aDepartment of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon, Maharashtra, 415 404, India
  • Namdeo Jadhav cDepartment of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra, 416 013, India
  • Dheeraj Randive bDepartment of Pharmaceutics, Rajarambapu College of Pharmacy, Kasegaon, Maharashtra, 415 404, India
  • Mangesh Bhutkar bDepartment of Pharmaceutics, Rajarambapu College of Pharmacy, Kasegaon, Maharashtra, 415 404, India
  • Rohankumar Chavan aDepartment of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon, Maharashtra, 415 404, India
  • Bajarang Kumbhar dDepartment of Biological Sciences, Sunandan Divatia School of Science, NMIMS University, Vile Parle (West), Mumbai, India

Keywords:

Blumea eriantha DC, Hair growth, HPLC, Molecular docking, GC/MS, Aayurveda

Abstract

In Aayurveda, Blumea eriantha DC has been used in the management of various diseases and is found to exhibit antioxidant and anti-hyperlipidemic, hypoglycemic, anti-diarrhoeal, larvicidal, antimicrobial properties. Objective The present study has focused on isolation of the active fraction from B. eriantha DC extract and to investigate its effect as a hair growth promoter along with identification of phytoconstituent(s) responsible for hair growth activity and its probable mechanism of action. Materials and methods Our work introduces an effective isolation protocol for the active fraction from B. eriantha DC extract using chromatographic techniques. Fraction A was isolated by using mobile phase toluene:acetone (9:1). In-vitro and in-vivo methods were executed for the evaluation of hair growth activity. Moreover, the docked conformations of the isolated phytoconstituent Dimethyl sulfone was compared to Minoxidil for selected proteins namely 2FGF, 2PVC and 4U7P. The PDB identifications 2PVC (DNMT3L recognizes unmethylated histone H3 lysine 4), 4U7P (Crystal structure of DNMT3A-DNMT3L complex and 2FGF (Human Basic Fibroblast Growth Factor) were downloaded from Protein Data Bank. Results The study data revealed that B. eriantha DC alcoholic extracts exhibited prominent hair growth activity and it was affirmed that Dimethyl sulfone a phyto-constituent isolated from B. eriantha DC alcoholic extract contributed for the same. Conclusion The findings strongly suggest hair growth promotion potential of the extract of B. eriantha DC. Keywords: Blumea eriantha DC, Hair growth, HPLC, Molecular docking, GC/MS, Aayurveda Abbreviations: BE, Blumea eriantha DC; AR Grade, Analytical grade; CC, Column chromatography; TLC, Thin layer chromatography; GC-MS, Gas chromatography-mass spectrometr; HPLC, High performance liquid chromatography; IAEC, Institutional animal ethical committee; SUK, Shivaji University, Kolhapur; RCSB-PDB, Research collaboratory structural bioinformatics protein data bank; SEm, Standard error mean; S.D, Standard deviation

Author Biography

Rohankumar Chavan, aDepartment of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon, Maharashtra, 415 404, India

eDepartment of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra, 416013, India

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