Preprint / Version 1

Exopolysaccharide Produced by Probiotic Bacillus albus DM-15 Isolated From Ayurvedic Fermented Dasamoolarishta: Characterization, Antioxidant, and Anticancer Activities

Authors

  • Annadurai Vinothkanna School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China
  • Ganesan Sathiyanarayanan Institute of Chemistry, University of Neuchâtel, Neuchâtel, Switzerland
  • Amit Rai Institute of Bioresources and Sustainable Development, Regional Centre, Gangtok, India
  • Krishnamurthy Mathivanan School of Minerals Processing and Bioengineering, Central South University, Hunan, China
  • Kandasamy Saravanan Department of Biochemistry, Bharathidasan University, Tiruchirappalli, India
  • Kumaresan Sudharsan Department of Chemistry, The Gandhigram Rural Institute (Deemed to be University), Dindigul, India
  • Palanisamy Kalimuthu Department of Chemistry, The Gandhigram Rural Institute (Deemed to be University), Dindigul, India
  • Yongkun Ma School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China
  • Soundarapandian Sekar Department of Biotechnology, Bharathidasan University, Tiruchirappalli, India

Keywords:

probiotics, Bacillus ablus, exopolysaccharides, characterization, antioxidant, anticancer potential

Abstract

An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus albus DM-15, isolated from the Indian Ayurvedic traditional medicine Dasamoolarishta. Gas chromatography-mass spectrophotometry and nuclear magnetic resonance (NMR) analyses revealed the heteropolymeric nature of the purified EPS with monosaccharide units of glucose, galactose, xylose, and rhamnose. Size-exclusion chromatography had shown the molecular weight of the purified EPS as around 240 kDa. X-ray powder diffraction analysis confirmed the non-crystalline amorphous nature of the EPS. Furthermore, the purified EPS showed the maximum flocculation activity (72.80%) with kaolin clay and emulsification activity (67.04%) with xylene. In addition, the EPS exhibits significant antioxidant activities on DPPH (58.17 ± 0.054%), ABTS (70.47 ± 0.854%) and nitric oxide (58.92 ± 0.744%) radicals in a concentration-dependent way. Moreover, the EPS showed promising cytotoxic activity (20 ± 0.97 μg mL–1) against the lung carcinoma cells (A549), and subsequent cellular staining revealed apoptotic necrotic characters in damaged A549 cells. The EPS purified from the probiotic strain B. albus DM-15 can be further studied and exploited as a potential carbohydrate polymer in food, cosmetic, pharmaceutical, and biomedical applications. Keywords: probiotics, Bacillus ablus, exopolysaccharides, characterization, antioxidant, anticancer potential

Author Biography

Annadurai Vinothkanna, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China

Department of Biotechnology, Bharathidasan University, Tiruchirappalli, India

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