Preprint / Version 1

Perilla pomace obtained from four different varieties have different levels and types of polyphenols and anti-allergic activity

Authors

  • Yuba Gaihre Saga Foods & Cosmetics Laboratory, Division of Research and Development Promotion, Saga Prefectural Regional Industry Support Center, 114 Yaemizo, Nabesima-machi, Saga, 849-0932 Japan
  • Akira Iwamoto Industrial Technology Center of Saga, 114 Yaemizo, Nabesima-machi, Saga, 849-0932 Japan
  • Shigeki Oogai Saga Foods & Cosmetics Laboratory, Division of Research and Development Promotion, Saga Prefectural Regional Industry Support Center, 114 Yaemizo, Nabesima-machi, Saga, 849-0932 Japan
  • Hiroshi Hamajima Saga Foods & Cosmetics Laboratory, Division of Research and Development Promotion, Saga Prefectural Regional Industry Support Center, 114 Yaemizo, Nabesima-machi, Saga, 849-0932 Japan
  • Keisuke Tsuge Industrial Technology Center of Saga, 114 Yaemizo, Nabesima-machi, Saga, 849-0932 Japan
  • Yasuo Nagata Saga Foods & Cosmetics Laboratory, Division of Research and Development Promotion, Saga Prefectural Regional Industry Support Center, 114 Yaemizo, Nabesima-machi, Saga, 849-0932 Japan
  • Teruyoshi Yanagita Saga Foods & Cosmetics Laboratory, Division of Research and Development Promotion, Saga Prefectural Regional Industry Support Center, 114 Yaemizo, Nabesima-machi, Saga, 849-0932 Japan

Keywords:

Anti-allergy, Perilla pomace, Apigenin, Luteolin, Rosmarinic acid, Degranulation

Abstract

Perilla frutescens (L.) Britton var. frutescens (egoma in Japan) is a traditional oilseed that has several varieties with different photoperiod responses. Although egoma pomace, industrial waste produced during oil extraction, is a rich source of macro- and micro-nutrients such as protein, fiber, minerals, and polyphenols, it has not yet been used for purposes other than livestock feeding. To find out a better use of perilla pomace and its function, we selected four varieties of egoma originating from different regions with different photoperiod responses: two varieties were from Japan, which are broadly cultivated for oilseed and are highly sensitive to light and temperature. The other two varieties from Nepal, which are tolerant to low light and low temperature. Rosmarinic acid-3-O-glucoside, rosmarinic acid, and apigenin-7-O-glucoside were detected as the main polyphenolic constituents in every variety, while apigenin and luteolin were present only in perilla pomace from Japan. In IgE-sensitized RBL-2H3 cells, polyphenols derived from two varieties of Japan suppressed degranulation of mast cells, but those derived from the two varieties of Nepal did not, indicating that apigenin and luteolin may be in part responsible for the anti-allergic response. In addition, it was found that proteins involved in the degranulation signaling pathway, such as PLCĪ³2, Syk, and Akt, were less phosphorylated in cells treated with the egoma pomace extracts of Japanese origin. Taken together, pomace from egoma varieties derived from different regions may differently modulate allergic response in part due to the difference in polyphenol composition and may be applied to develop nutraceuticals and functional foods fortified with anti-allergic properties. Keywords: Anti-allergy, Perilla pomace, Apigenin, Luteolin, Rosmarinic acid, Degranulation

Author Biographies

Yasuo Nagata, Saga Foods & Cosmetics Laboratory, Division of Research and Development Promotion, Saga Prefectural Regional Industry Support Center, 114 Yaemizo, Nabesima-machi, Saga, 849-0932 Japan

Center for Industry, University and Government Cooperation, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8535 Japan

Teruyoshi Yanagita, Saga Foods & Cosmetics Laboratory, Division of Research and Development Promotion, Saga Prefectural Regional Industry Support Center, 114 Yaemizo, Nabesima-machi, Saga, 849-0932 Japan

Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo, Honjo-machi, Saga, 840-8502 Japan

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