Preprint / Version 1

Anti-Obesity Action of Boerhavia diffusa in Rats against High-Fat Diet-Induced Obesity by Blocking the Cannabinoid Receptors

Authors

  • Mohammad Khalid Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia; [email protected] (M.H.A.); [email protected] (A.I.F.); [email protected] (T.M.A.)
  • Mohammed Alqarni Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia; [email protected] (M.H.A.); [email protected] (A.I.F.); [email protected] (T.M.A.)
  • Ambreen Shoaib Department of Clinical Pharmacy, College of Pharmacy, Jazan University, P.O. Box 114, Jazan 45142, Saudi Arabia; [email protected] (A.S.); [email protected] (S.A.)
  • Shadma Wahab Department of Pharmacognosy, College of Pharmacy, King Khalid University, P.O. Box 960, Abha 61421, Saudi Arabia; [email protected]
  • Ahmed Foudah Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia; [email protected] (M.H.A.); [email protected] (A.I.F.); [email protected] (T.M.A.)
  • Tariq Aljarba Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia; [email protected] (M.H.A.); [email protected] (A.I.F.); [email protected] (T.M.A.)
  • Juber Akhtar Department of Pharmaceutics, Faculty of Pharmacy, Integral University, Lucknow 226026, India; [email protected]
  • Mubarak Alamri Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia; [email protected]
  • Sarfaraz Ahmad Department of Clinical Pharmacy, College of Pharmacy, Jazan University, P.O. Box 114, Jazan 45142, Saudi Arabia; [email protected] (A.S.); [email protected] (S.A.)

Keywords:

Boerhavia diffusa, boeravinone B, punarnavine, Eupalitin, cannabinoid receptors, obesity

Abstract

Obesity, type 2 diabetes, and cardiovascular illnesses have known risk factors in the pathophysiology of an unhealthy diet. Obesity now affects almost a third of the world’s population and is widely seen as a side effect of the Industrial Revolution. The current study aimed to determine natural phytoconstituents that have a significant role in the management of obesity. In this view, we have selected the plant Boerhavia diffusa which has different pharmacological actions and is traditionally used to treat sickness caused by lifestyle modification. The methanolic extract of the plant material was prepared and then further fractionated by means of solvents (n-hexane, chloroform, n-butanol, and water). The absorption, distribution, metabolism, excretion, and toxicity (ADMET) analysis was done by taking the active constituent of the plant (Punarnavine, Boeravinone B, and Eupalitin). The molecular docking analysis of these compounds is also performed by targeting the cannabinoid receptor (CR). Structural analysis of the best complex was done using the Discovery Studio visualizer tool. High-performance thin-layer chromatography (HPTLC) analysis was done by using a solvent system (chloroform and methanol in a ratio of 8:2). The in vivo study was done on the Sprague–Dawley (SD) rats treated with a high-fat diet to induce obesity and different parameters such as body weight, behavioral activity, organ fat pad weight, lipid profile, and liver biomarkers (AST, ALT, BUN, and creatinine) were estimated. The result of the study suggested that the phytoconstituents of B. diffusa upon molecular docking revealed the possible binding mechanisms with the CR and thus show potent anti-obesity action. Keywords: Boerhavia diffusa, boeravinone B, punarnavine, Eupalitin, cannabinoid receptors, obesity

Downloads