Preprint / Version 1

Anti-hyperglycemic, anti-hyperlipidemic, and anti-inflammatory effect of the drug Guggulutiktaka ghrita on high-fat diet-induced obese rats

Authors

  • Samreen Sheik aDepartment of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
  • Pugazhandhi Bakthavatchalam bDepartment of Anatomy, Melaka Manipal Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
  • Revathi Shenoy aDepartment of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
  • Basavaraj Hadapad cDivision of Ayurveda, Centre for Integrative Medicine and Research, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
  • Deepak M dDepartment of Pathology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
  • Monalisa Biswas aDepartment of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
  • Varashree Suryakanth aDepartment of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India

Keywords:

Guggulutikthaka gritha, High fat diet, Hyperglycemia, Hyperlipidemia, Inflammation

Abstract

Ayurveda is a holistic system of medicine and describes a vast array of herbs and herbal mixtures that are been demonstrated to possess efficacy in research investigations. Guggulutikthaka gritha (GTG) is one such drug evaluated for its role in skin and bone diseases. Objective In the current study, the hypoglycemic, hypolipidemic, and anti-inflammatory effect of the drug GTG was studied with the scope to treat dyslipidemia and thereby reduce the risk of cardiovascular disease. Materials and method The animals (Wistar rats) were fed a high-fat diet and dyslipidemia was induced. The control group was provided with a normal chow diet and had free access to water. The treatment with the drug GTG was given for 21 days after confirming dyslipidemia. The blood glucose was measured immediately using a glucometer. The serum was analyzed for lipid profile and Vascular Cell Adhesion Molecule – 1(VCAM 1) by ELISA method before and after treatment. The histopathology of the heart and liver was also performed. Results The abnormal change in lipid profile, blood glucose, and inflammatory marker along with the accumulation of intracellular fats in the arteries of the heart and liver confirmed dyslipidemia. A significant reduction in serum lipid profile (p < 0.05), blood glucose (p < 0.05), and VCAM 1 (p < 0.05) was noted after the treatment with significant histopathological changes in arteries of the heart and liver. Conclusion The study provides scientific validation on the drug GTG being effective in hyperglycemia, hyperlipidemia, and inflammation in dyslipidemia. Keywords: Guggulutikthaka gritha, High fat diet, Hyperglycemia, Hyperlipidemia, Inflammation Abbreviations: ASCVD, Atherosclerotic cardiovascular disease; CVD, Cardiovascular disorders; CRP, C-reactive protein; DM, diabetes mellitus; GTG, Guggulutikthaka gritha; HDL, High-Density Lipoprotein; HFD, High fat diet; ICAM-1, Intracellular adhesion molecule 1; IL-1, IL- 6, IL-10, Interleukins; IR, Insulin resistance; LDL, Low-density lipoprotein; LPL, Lipoprotein lipase; OECD, Organization for economic co-operative and development; oxLDL, Oxidized LDL; ROS, Reactive Oxygen Species; TG, Triglycerides; TLR, Toll-like receptors; TNF-α, Tumor necrosis factor; VCAM 1, Vascular cell adhesion molecule – 1; VLDL, Very-low-density lipoprotein

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