Preprint / Version 1

Role of semi-purified andrographolide from Andrographis paniculata extract as nano-phytovesicular carrier for enhancing oral absorption and hypoglycemic activity

Authors

  • Vinod Verma aDepartment of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh 786004, India
  • Md Zaman aDepartment of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh 786004, India
  • Shekhar Verma cUniversity College of Pharmacy, Pt. Deendayal Upadhyay Health, Science and Aayush University Raipur-492001, Chhattisgarh, India
  • Santosh Verma dSchool of Chemistry and Chemical Engineering, Yulin University, Yulin 719000, China
  • Khomendra Sarwa fDepartment of Pharmacy, Government Girl's Polytechnic, Raipur 492001, India

Keywords:

Andrographis paniculata (Burm. f.) Nees, andrographolide, hypoglycemic activity, liquid dispersion, nano-phyto vesicle, soya phosphatidylcholine

Abstract

Objective Andrographis paniculata is a well-known medicinal plant in Southeast Asia, India and China. The plant contains andrographolide (AN), a very important phytochemical used in various health problems. However, AN is low in oral absorption bioavailability of AN due to the rapid clearance and high protein binding capacity. Methods The present study was aimed to develop a nano-phytovesicular formulation of semi-purified AN extracts from a naturally occurring phospholipid (soya phosphatidylcholine) in order to increase the oral absorption and antihyperglycemic activity in rats. Results The nano-phyto vesicle of semi-purified AN extracts equivalent to 25 mg /kg AN significantly protected the hyperglycemic condition of rats. The in vitro and in vivo experiments results proved that the nano- phytovesicular system of plant extracts containing AN produced better oral absorption, bioavailability and improved antihyperglycemic activity compared with that of free AN at dose of 50 mg/kg. Conclusion Hence, the prepared semi-purified extract nano-phytovesicular system is helpful in solving the problem of rapid clearance of AN. Keywords: Andrographis paniculata (Burm. f.) Nees, andrographolide, hypoglycemic activity, liquid dispersion, nano-phyto vesicle, soya phosphatidylcholine

Author Biographies

Vinod Verma, aDepartment of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh 786004, India

bState Food and Drug Testing Laboratory, Raipur 492001, India

Santosh Verma, dSchool of Chemistry and Chemical Engineering, Yulin University, Yulin 719000, China

eShaanxi Key Laboratory of Low Metamorphic Coal Clean Utilization, Yulin University, Yulin 719000, China