Toxicity Assessment of Curculigo orchioides Leaf Extract Using Drosophila melanogaster: A Preliminary Study
Authors
Sharanya Kushalan
Nitte (Deemed to Be University), Nitte University Centre for Science Education and Research (NUCSER), Division of Bioresource and Biotechnology, Kotekar-Beeri Road, Deralakatte, Mangaluru 575018, India
Leonard D’Souza
Nitte (Deemed to Be University), Nitte University Centre for Science Education and Research (NUCSER), Division of Environmental Health and Toxicology, Kotekar-Beeri Road, Deralakatte, Mangaluru 575018, India
Khyahrii Aloysius
Nitte (Deemed to Be University), Nitte University Centre for Science Education and Research (NUCSER), Division of Bioresource and Biotechnology, Kotekar-Beeri Road, Deralakatte, Mangaluru 575018, India
Anurag Sharma
Nitte (Deemed to Be University), Nitte University Centre for Science Education and Research (NUCSER), Division of Environmental Health and Toxicology, Kotekar-Beeri Road, Deralakatte, Mangaluru 575018, India
Smitha Hegde
Nitte (Deemed to Be University), Nitte University Centre for Science Education and Research (NUCSER), Division of Bioresource and Biotechnology, Kotekar-Beeri Road, Deralakatte, Mangaluru 575018, India
Curculigo orchioides is used in Indian and Chinese traditional medicinal systems for various health benefits. However, its toxicological effects are mostly unknown. This study assesses the potential toxicity of aqueous leaf (A.L.) extract of C. orchioides using Drosophila melanogaster as an experimental model. Preliminary phytochemical tests were followed by the Fourier transform infrared (FTIR) tests to identify the functional group in the A.L. extract of C. orchioides. Drosophila larvae/adults were exposed to varying concentrations of C. orchioides A.L. extract through diet, and developmental, lifespan, reproduction, and locomotory behaviour assays were carried out to assess the C. orchioides toxicity at organismal levels. The cellular toxicity of A.L. extract was examined by analysing the expression of heat shock protein (hsps), reactive oxygen species (ROS) levels, and cell death. The FTIR analysis showed the presence of functional groups indicating the presence of secondary metabolites like saponins, phenolics, and alkaloids. Exposure to A.L. extract during development resulted in reduced emergence and wing malformations in the emerged fly. Furthermore, a significant reduction in reproductive performance and the organism’s lifespan was observed when adult flies were exposed to A.L. extract. This study indicates the adverse effect of C. orchioides A.L. extract on Drosophila and raises concerns about the practice of indiscriminate therapeutic use of plant extracts.
Keywords: Curculigo orchioides, leaf extract, development, wing defect, reproductive toxicity, Drosophila
Author Biographies
Sharanya Kushalan, Nitte (Deemed to Be University), Nitte University Centre for Science Education and Research (NUCSER), Division of Bioresource and Biotechnology, Kotekar-Beeri Road, Deralakatte, Mangaluru 575018, India
Data curation, Methodology, Writing – original draft
Leonard D’Souza, Nitte (Deemed to Be University), Nitte University Centre for Science Education and Research (NUCSER), Division of Environmental Health and Toxicology, Kotekar-Beeri Road, Deralakatte, Mangaluru 575018, India
Methodology, Software, Visualization
Khyahrii Aloysius, Nitte (Deemed to Be University), Nitte University Centre for Science Education and Research (NUCSER), Division of Bioresource and Biotechnology, Kotekar-Beeri Road, Deralakatte, Mangaluru 575018, India
Methodology
Anurag Sharma, Nitte (Deemed to Be University), Nitte University Centre for Science Education and Research (NUCSER), Division of Environmental Health and Toxicology, Kotekar-Beeri Road, Deralakatte, Mangaluru 575018, India
Smitha Hegde, Nitte (Deemed to Be University), Nitte University Centre for Science Education and Research (NUCSER), Division of Bioresource and Biotechnology, Kotekar-Beeri Road, Deralakatte, Mangaluru 575018, India
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