Preprint / Version 1

Root Bark Extract of Oroxylum indicum Vent. Inhibits Solid and Ascites Tumors and Prevents the Development of DMBA-Induced Skin Papilloma Formation

Authors

  • Seema Menon Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India
  • Jawaher Albaqami Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
  • Hamida Hamdi Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
  • Lincy Lawrence Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India
  • Menon Divya Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India
  • Liya Antony Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India
  • Jose Padikkala Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India
  • Shaji Mathew Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India
  • Arunaksharan Narayanankutty Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India

Keywords:

Oroxylum indicum, antioxidant activity, dimethyl benz(a) anthracene, skin papilloma, antitumor activity

Abstract

Oroxylum indicum is a traditionally used plant in Ayurvedic and folk medicines. The plant is useful for the management of gastrointestinal diseases as well as skin diseases. In the present study, we analyzed the antitumor potential of O. indicum in Dalton’s lymphoma ascites tumor cells (DLA) and Ehrlich ascites carcinoma (EAC)-induced solid and ascites tumors. Further, the potential of O. indicum extract (OIM) on skin papilloma induction by dimethyl benz(a) anthracene (DMBA) and croton oil was evaluated. The chemical composition of the extract was analyzed using UPLC-Q-TOF-MS. The predominant compounds present in the extract were demethoxycentaureidin 7-O-rutinoside, isorhamnetin-3-O-rutinoside, baicalein-7-O-glucuronide, 5,6,7-trihydroxyflavone, 3-Hydroxy-3′,4′,5′-trimethoxyflavone, 5,7-dihydroxy-3-(4-methoxyphenyl) chromen-4-one, and 4′-Hydroxy-5,7-dimethoxyflavanone. Treatment with high-dose OIM enhanced the percentage of survival in ascites tumor-bearing mice by 34.97%. Likewise, high and low doses of OIM reduced the tumor volume in mice by 61.84% and 54.21%, respectively. Further, the skin papilloma formation was brought down by the administration of low- and high-dose groups of OIM (by 67.51% and 75.63%). Overall, the study concludes that the Oroxylum indicum root bark extract is a potentially active antitumor and anticancer agent. Keywords: Oroxylum indicum, antioxidant activity, dimethyl benz(a) anthracene, skin papilloma, antitumor activity

Author Biographies

Seema Menon, Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India

Conceptualization, Methodology, Investigation, Writing – original draft

Jawaher Albaqami, Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia

Methodology, Formal analysis, Investigation, Writing – original draft, Writing – review & editing

Hamida Hamdi, Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia

Conceptualization, Methodology, Formal analysis, Investigation, Writing – original draft, Writing – review & editing, Funding acquisition

Lincy Lawrence, Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India

Methodology, Validation, Formal analysis, Resources, Writing – original draft, Visualization

Menon Divya, Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India

Validation, Formal analysis, Resources, Data curation, Writing – original draft, Writing – review & editing

Liya Antony, Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India

Formal analysis, Writing – original draft, Visualization

Jose Padikkala, Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India

Conceptualization, Methodology, Validation, Writing – review & editing, Project administration

Shaji Mathew, Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India

Methodology, Software, Validation, Formal analysis, Investigation, Writing – original draft, Visualization

Arunaksharan Narayanankutty, Department of Biochemistry and Plant Biotechnology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, India

Conceptualization, Methodology, Software, Formal analysis, Data curation, Writing – original draft, Project administration

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