Comparative Evaluation of Antimicrobial Efficacy of Herbal Formulations of Septilin and Triphala with Conventional 2% Chlorhexidine on Root Canal and Oral Commensal Bacteria using Kirby Bauer Method: An in-vitro Study
Authors
Shadab Ahmed
Department of Conservative Dentistry and Endodontics, Sri Siddhartha Dental College and Hospital, Tumakuru, Karnataka, India
Kamil Shahnawaz
Department of Conservative Dentistry and Endodontics, Community Health Centre, Munger, Bihar, India
Tapan Mandal
Department of Conservative Dentistry and Endodontics, Hazaribag College of Dental Sciences, Hazaribag, Jharkhand, India
Mamnoon Ghafir
Department of Conservative Dentistry and Endodontics, Institute of Dental Sciences, Bareilly, Uttar Pradesh, India
Shiva Gummaluri
Department of Periodontology and Implantology, Sree Sai Dental College and Research Institute, Srikakulam, Andhra Pradesh, India
Gaurav Vishal
Department of Oral and Maxillofacial Surgery, Institute of Dental Sciences, Bareilly, Uttar Pradesh, India
Endodontic flareups are always very difficult to treat because of reasons like drug resistance previously sodium hypochlorite and chlorhexidine have been used in the literature but with advancements newer materials like herbal products have been tried for this purpose. Thus, present vitro study was conducted to comparatively evaluate the efficacy of herbal formulations on commensals of oral cavity and root canal.
Materials and Methods:
In this in vitro study antimicrobial efficacy of chlorhexidine 2%, Septilin and Triphala wer evaluated with the help of Kirby Bauer test. The microorganisms used were Staphylococcus aureus and Escherichia coli. The samples were placed in standardized wells and were subjected to incubation. Subsequently zones of inhibition were measured with the help of a caliper. One-factor analysis of variance, Tukey's least significant difference post hoc test and Student's independent t-test were performed to find a significant difference (P < 0.05) in the two groups.
Results:
For chlorhexidine in S. aureus mean antibacterial efficacy was 11.10 ± 1.25 and in E. coli 10.0 ± 1.33. The value for S. aureus was lesser in Triphala with a value of 10.35 ± 1.63 and in E. coli mean value was 9.05 ± 1.27. For Septilin the mean for S. aureus was 10.40 ± 1.04, and the mean antibacterial efficacy in E. coli was 9.65 ± 1.38.
Conclusion:
Septilin and Triphala showed remarkable efficacy concerning zones of inhibition. These herbal formulations have tremendous potential to be used as adjuncts to traditional disinfection modalities though it has scope of further research.
Keywords: Antimicrobial efficacy, escherichia coli, kirby bauer test, septilin, staphylococcus aureus, triphala
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