Herbal Honey Preparations of Curcuma Xanthorriza and Black Cumin Protect against Carcinogenesis through Antioxidant and Immunomodulatory Activities in Sprague Dawley (SD) Rats Induced with Dimethylbenz(a)anthracene
Authors
Titiek Hidayati
Department of Public Health and Family Medicine, Faculty of Medicine and Health Science, Universitas Muhammadiyah Yogyakarta, Yogyakarta 55252, Indonesia
Indrayanti Indrayanti
Department of Anatomical Pathology, Faculty of Medicine and Health Science, Universitas Muhammadiyah Yogyakarta, Yogyakarta 55252, Indonesia
Endang Darmawan
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta 55252, Indonesia
Akrom Akrom
Department of Pharmacology and Clinical Pharmacy, Master Pharmacy Degree Program, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta 55252, Indonesia
Keywords:
herbal honey, curcuma xanthorriza, black cumin seed, chemopreventive, antioxidants, immunomodulatory
Abstract
Background: Traditionally, Curcuma xanthorriza (CX), black cumin seed (BC), and honey have been used by the Indonesian people as medicinal ingredients to treat various health symptoms. CX extracts and BC have been proven in the laboratory as chemopreventive agents, antioxidants, and immunomodulators. In this study, we developed CX extract, BC oil, and honey into herbal honey preparations (CXBCH) and hypothesized that the preparations show chemopreventive activity. The purpose of the study was to determine the CXBCH potential as chemopreventive, antioxidant, and immunomodulatory. Method: In this experimental laboratory research, antioxidant, immunomodulatory, and cytotoxic activities were tested on human mammary cancer cell lines (T47D cells) while the chemopreventive activity of the CXBCH preparations on Sprague Dawley (SD) rats induced with dimethylbenzene(a)anthracene (DMBA). Results: CXBCH preparations demonstrated immunomodulatory, antioxidant, and cytotoxic activities in T47D, Hela, and HTB-183 cells and in DMBA-induced SD rats, as the preparations inhibited tumor nodule formation, increased the number of CD4, CD8 and CD4CD25 cells, and glutathione-S-transferase (GST) activity, and decreased serum NO levels. Conclusions: CXBCH preparations display chemopreventive, antioxidant, and immunomodulatory properties.
Keywords: herbal honey, curcuma xanthorriza, black cumin seed, chemopreventive, antioxidants, immunomodulatory
Author Biographies
Titiek Hidayati, Department of Public Health and Family Medicine, Faculty of Medicine and Health Science, Universitas Muhammadiyah Yogyakarta, Yogyakarta 55252, Indonesia
Methodology, Validation, Formal analysis, Investigation, Data curation, Writing – original draft, Writing – review & editing, Supervision, Funding acquisition
Indrayanti Indrayanti, Department of Anatomical Pathology, Faculty of Medicine and Health Science, Universitas Muhammadiyah Yogyakarta, Yogyakarta 55252, Indonesia
Akrom Akrom, Department of Pharmacology and Clinical Pharmacy, Master Pharmacy Degree Program, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta 55252, Indonesia
Conceptualization, Methodology, Validation, Investigation, Data curation, Writing – original draft, Writing – review & editing, Project administration
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